肿瘤靶向多肽生物淘选技术研究进展

Advances in the research and development of phage display peptide library technology for biopanning of tumor targeting peptides

生物淘选肿瘤或癌细胞的各种特异性靶向多肽是探索肿瘤早期诊断和治疗方法的有效途径.特异性靶向多肽既可用于探测肿瘤细胞表型特征,又可将其与抗癌药物结合进行靶向治疗,同时还可作为体内肿瘤的成像制剂,用于肿瘤及肿瘤细胞微转移的早期探测.噬菌体展示肽库技术能够有效地进行生物淘选或识别出与癌或肿瘤细胞某一靶点连结的,具有特异性、高亲和性的多肽.近年来,利用体内或体外噬菌体展示肽库技术已成功地淘选或识别出一些癌细胞的靶向多肽.本文就这些方面的研究进展进行了简要阐述,重点介绍了癌或肿瘤细胞靶向多肽的体外和体内生物淘选最新技术和临床应用.

Bioparming tumor-specific or cancer-specific binding peptide as targeting factor is one of the effective methods for earlier diagnosis and treatment of tumor. Tumor-specific binding peptides can be used to probe the tumor cell surface phenotype and customize treatment accordingly by binding to an anticancer drug. Additionally, these targeting peptides can be used as in vivoimaging agents that allow for earlier detection of tumors and micrometastasis. Phage display is a powerful technique for the isolation of peptides that bind to a particular target with high affinity and specificity. The biopanning of intact cancer cells or tumors in animals can be used to isolate peptides that bind to cancer-specific cell surface biomarkers. Over the past 10 years, unbiased biopanning of phage-displayed peptide libraries has generated a suite of cancer targeting peptidic ligands. This review discusses the identification of surface features on cancer or tumor cells and methods of biopanning with phage display peptide technology in vitro and in vivo, and highlights advances in the research and development of screening of cancer or tumor cells targeting peptides as well as the use of the isolated peptides in clinical applications.