饲料中添加虾青素对红罗非鱼各组织类胡萝卜素含量和沉积率的影响

Effect of dietary astaxanthin on carotenoid content and deposition rate in tissues of red tilapia

通过在饲料中添加不同含量虾青素,测定粉白、红斑、黑斑3种体色红罗非鱼各组织(皮肤、鳍、肌肉、眼球、鳃)类胡萝卜素含量的变化,分析不同虾青素含量对红罗非鱼不同组织类胡萝卜素含量的影响,从而推断类胡萝卜素在不同组织间的表达和沉积方式。结果显示,虾青素添加量为400 mg/kg时,红罗非鱼皮肤、鳍、肌肉、眼球和鳃中的类胡萝卜素含量与对照组相比均明显提高,体色明显改善,其中黑斑红罗非鱼鳃部类胡萝卜素沉积比对照组有显著提高(P<0.05)。而当虾青素添加量为800 mg/kg时,粉白红罗非鱼鳍上和鳃部及黑斑红罗非鱼鳃部类胡萝卜素沉积比对照组有显著提高(P<0.05),但3种体色红罗非鱼一些组织器官类胡萝卜素含量相对对照组及虾青素添加量为400 mg/kg时有所下降,红罗非鱼体色变化不明显。虾青素含量对红罗非鱼各组织类胡萝卜素总含量有显著影响(P<0.01);而红罗非鱼体色对类胡萝卜素总含量影响不显著(P>0.05);虾青素和体色交互作用对类胡萝卜素含量有较显著影响(P<0.05)。聚类分析表明,类胡萝卜素在红罗非鱼的鳍中沉积率最高,其次为鳃,再次为眼球,皮肤和肌肉沉积率最低。

This study was designed to measure the changes of the carotenoid content in tissues（ skin,fin, muscle,eyeball,gill） of pink,red spots and black spots red tilapia,and thus to infer carotenoid expression and sedimentary method between different tissues by adding different amounts of astaxanthin in the feed. The results show that when the dosage of dietary astaxanthin is 400 mg / kg,the carotenoid content in tissues of red tilapia was significantly increased and the body color of red tilapia was significantly improved compared with the control group,while the carotenoid sedimentary amount in the gill of dark spots red tilapia has improved significantly compared to the control group（ P 〈0. 05）. When the dosage of dietary astaxanthin is 800 mg / kg,the carotenoid sedimentary amount in the fin of pink red tilapia and the gill of dark spots red tilapia has improved significantly compared to the control group（ P 〈0. 05）,but the other tissues of the carotenoid content of other tissues showed a downward trend compared to the control group and the dosage of 400 mg / kg, and the body color of the red tilapia did not change significantly. Astaxanthin content has a significant impact on the total carotenoid content of red tilapia tissues（ P 〈0. 01）. The impact of the body color of red tilapia on total carotenoid content was not significant（ P 〈0. 05）. The interaction between astaxanthin and body color has a more significant effect on carotenoid content（ P 〈0. 05）. Cluster analysis showed that deposition rate in fin is the highest,followed by gill,and the next is the eyeball,deposition rate in skin and muscle is the lowest.