摘要
目的:探讨姜黄素对氧化应激损伤后的海马神经元血红素加氧酶-1(heme oxygenase-1,HO-1)的表达影响。方法:将培养的原代海马神经元,分为6组:空白对照组、损伤对照组、姜黄素不同剂量治疗组(2.5μmol/L,5μmol/L,10μmol/L,20μmol/L)。利用1 mmol/L的H2O2处理海马神经元1 h,制作神经元氧化应激损伤模型。按照以上分组情况加入等浓度的姜黄素培养液,37℃、5%CO2孵箱孵育6 h,采用倒置相差显微镜观察细胞的形态变化;免疫荧光、PCR半定量检测细胞内HO-1的表达。结果:①5μmol/L组、10μmol/L组细胞形态变化不明显;损伤对照组和2.5μmol/L组细胞损伤明显;②损伤对照组与空白对照组比较,P<0.05;损伤对照组与2.5μmol/L组、20μmol/L组两两比较差异无统计学意义(P>0.05);5μmol/L组、10μmol/L组与损伤对照组比较,差异有统计学意义(P<0.05);PCR检测结果提示20μmol/L组比2.5μmol/L组HO-1的表达量减少。结论:姜黄素对氧化应激损伤海马神经元的保护机制可能是通过上调HO-1的表达实现的。
Objective： To explore the effect of curcumin on heme oxygenase-1 expression in hippocampal neurons following oxidative stress. Methods： The primary cultured hippocampal neurons were divided into 6 groups ：the blank group, injury group, and 4 different doses of curcumin treatment groups （2.5 μmol/L, 5 μmol/L , 10 μmol/L and 20 μ mol/L groups）. Oxidative stress model was established by incubating hippocampal neurons with H2O2 （1 retool/L） for 1 hour. Different levels of curcumin were added to oxidative damaged neurons and then these neurons were cultured in incubator for 6 hours. The morphology of neurons were observed and the expression of HO-! were detected by immunofluorescence and PCR. Results： （1）Neuron morphology in 5 p, mol/L and 10μmol/L group had no evident alteration.Axons of neuron in injury group and 2.5 μmol/L group were injured obviously. （2）Compared injury group with control group, the difference of expression of HO-1 was significant （P 〈 0.05）. No significant difference was observed among injury group, 2.5 μmol/L group,and 20 μmol/L group （P 〈 0.05）. HO-1 expression in 5 μmol/L group and 10 μmol/L group were strikingly enhanced （P 〈 0.05）. There was no difference of HO-1 expression among 20 μmol/L group, injury group and 2.5 μmol/L group （P 〉 0.05）. Conclusion： The protective effects of curcumin on oxidative stress in hippcampal neurons maight be through the up-regulation of HO-1.
出处
《泸州医学院学报》
2014年第3期247-252,共6页
Journal of Luzhou Medical College
基金
泸州市科技局重点项目[2012-S-37(29/29)]
