清开灵注射液对金黄色葡萄球菌细菌生物膜的影响

Influence of Qingkailing Injection on biofilm of Staphylococcus aureus

目的 观察清开灵注射液对金黄色葡萄球菌细菌生物膜的影响,研究其抗菌机制.方法 96孔板内培养金黄色葡萄球菌的形成期及成熟期细菌生物膜,给药后微生物活性检测试剂盒-水溶性四唑盐染色,酶标仪测定450 nm处光密度值检测生物膜内活菌量.数控剪切流活细胞自动分析平台实时观察药物对成熟细菌生物膜的破坏作用;扫描电子显微镜观察给药后成熟细菌生物膜结构和细菌形态的改变.结果 清开灵注射液（22 500 ～ 352 mg/L）能减少形成期生物膜内活菌量,清开灵注射液（22 500～1 406 mg/L）能减少成熟细菌生物膜内活菌量,结果具有统计学意义（P＜0.05）.数控剪切流活细胞自动分析平台结果表明,清开灵注射液能破坏成熟金黄色葡萄球菌细菌生物膜的结构.扫描电子显微镜结果表明,清开灵注射液使细菌生物膜变得疏松,菌体大小不一.结论 清开灵注射液抑制金黄色葡萄球菌细菌生物膜的形成并破坏其成熟细菌生物膜,具有抗金黄色葡萄球菌细菌生物膜的作用.

Objective To observe the influence of Qingkailing Injection on biofilm of Staphylococcus aureus （S. aureus ）, and study its antibacterial mechanism. Methods The biofilm of S. aureus was cultured on 96-well plate at formation stage and mature stage. The amount of living S. aureus in biofilm was determined by using Microbial Viability Assay Kit-WST dyeing after OD value obtained from 450 nm by using Microplate Reader. The destructive effect of Qingkailing Injection on measure biofilm was observed by using BioFlux biofilm system, and the changes of mature biofilm structure and S. aureus morphology after medication were observed by using scanning electron microscope. Results Qingkailing Injection reduced the amount of living S. aureus in biofilm at formation stage （22 500 mg/L - 352 mg/ L） , and reduced the amount of the amount of living S. aureus in biofilm at mature stage （22 500 mg/ L - 1 406 mg/L, P 〈 0.05 ）. The results of BioFlux biofilm system showed that Qingkailing Injection destructed the structure of measure biofilm of S. aureus, and the results of scanning electron microscope showed that Qingkailing Injection induced biofilm loose and S, aureus in Qingkailing Injection can inhibit the biofilm formation and destruct mature the anti-effect on the biofilm of S. aureus different sizes. Conclusion biofilm of S. aureus, so it has