维甲酸诱导的基因RA109染色体定位和蛋白质定位

Chromosome locus of ATRAinduced gene RA109 and intracellular localization of RA109 protein

目的 确定全反式维甲酸诱导肺腺癌细胞系GLC 82表达上调的基因RA10 9在染色体上的位置及其编码蛋白在细胞中的位置。方法 应用辐射杂交细胞系 (RH)定位的方法进行染色体定位 ,应用绿色荧光蛋白 (GFP)基因作为报告基因 ,结合荧光显微摄影进行蛋白质定位。结果 将RA10 9基因定位在 5号染色体长臂 5q35 ,RA10 9蛋白定位在细胞核。结论 RH定位是一个快速、简捷、精确的染色体定位方法 ,以此方法可将RA10 9定位在 5号染色体长臂的一个疾病基因富含区。利用GFP进行蛋白质定位简单明了 ,以此可将RA10 9蛋白定位在细胞核这个重要的细胞结构中。

Objective To define chromosome locus of ATRAinduced gene RA109 and intracellular localization of its encoding protein. Methods Chromosome locus was defined by radiation hybrid mapping method and protein localization by green fluorescence protein(GFP) as reporter molecule. Results RA109 gene was mapped to chromosome 5q35 and its protein was localized in cell nucleus. Conclusion RA109 gene is mapped by radiation hybrid technique to a chromosome region that is rich in diseaserelated genes. The RA 109encoding protein is a nucleoprotein.