鼻咽癌染色体11q13等位基因杂合性缺失的研究

Study of loss of heterozygosity at chromosome 11q13 in nasopharyngeal carcinoma

目的 对鼻咽癌中染色体 11q13上的 4个位点进行微卫星多态性分析 ,明确这些位点染色体等位基因杂合性丢失的情况。方法 采用显微切割的方法获取较纯的肿瘤组织 ,然后用PCR的方法以PYGM、D11S4946、D11S44 9和INT 2为引物 ,对 38例鼻咽癌进行微卫星序列分析。结果 38例鼻咽癌组织中 ,至少有一个位点出现杂合性缺失者 36例 ,占 94 7%。其中D11S4946杂合性缺失的频率最高 ,占 78 8% (2 6 / 33) ,其余的引物分别为 :INT 2占 5 1 5 % (17/ 33) ,PYGM占 45 5 % (15 / 33) ,D11S44 9占 45 7% (16 / 35 )。结论 鼻咽癌染色体 11q13区发生高频率杂合性缺失 ,提示缺失区域可能存在与鼻咽癌发生有关的抑癌基因。

Objective To analyze the loss of heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC). Methods Relatively pure tumor tissues were obtained by microdissetion. PCR was used to perform microsatellite analysis of NPC samples using D11S4946, PYGM, D11S449 and INT2 as primers, which span the MEN1 gene. Results Thirtysix of 38 cases studied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (788%). The LOH frequency was 515% for INT2, 455% for PYGM and 457% for D11S449, respectively. [WT5”HZ]Conclusion The high frequency of loss of heterozygosity at 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.