多囊卵巢综合征大鼠子宫内膜葡萄糖转运蛋白-4和胰岛素生长因子-1表达的研究被引量：3

Expression of glucose transporter protein 4 and insulin growth factor-1 in endometrial of rats with polycystic ovary syndrome

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摘要目的通过观察多囊卵巢综合征(PCOS)大鼠子宫内膜葡萄糖转运蛋白-4(GLUT-4)和胰岛素样生长因子(IGF-1)的表达以及应用二甲双胍后子宫内膜GLUT-4和IGF-1表达的改变。探讨影响PCOS大鼠子宫内膜容受性可能的机制。方法选取6周龄清洁级雌性Wistar大鼠70只,按体质量计算分为对照组20只、模型组50只。应用来曲唑灌胃建立PCOS大鼠模型,造模成功后,模型组抽取40只分取二甲双胍治疗组20只和PCOS组20只分别喂服二甲双胍和0.9%氯化钠注射液。14d后麻醉大鼠并取材,光镜观察子宫内膜的情况,免疫组织化学法测定3组子宫内膜GLUT-4和IGF-1的表达水平。结果 PCOS组大鼠子宫内膜腺上皮和间质中GLUT-4蛋白表达明显低于对照组(P<0.05),IGF-1的表达明显高于对照组(P<0.05)。治疗组GLUT-4表达较PCOS组显著增高(P<0.05)但仍低于对照组(P>0.05),治疗组IGF-1表达比PCOS组降低(P<0.05)与对照组相比仍然较高(P<0.05)。结论 PCOS大鼠子宫内膜GLUT-4和IGF-1均与对照组有明显的差异。通过二甲双胍治疗后GLUT-4和IGF-1的变化明显接近正常对照组,说明GLUT-4和IGF-1与PCOS子宫内膜改变有相关性。 Objective To observe the expression of glucose transporter proteins 4（GLUT-4）and insulinlike growth factor-1（IGF-1）of endometrium in rats with polycystic ovarian syndrome（PCOS）,and the change of GLUT-4and IGF-1of endometrium in rats after therapy with metformin.To investigate the mechanisms affecting endometrial receptivity of rats with PCOS.Methods To set up an animal model by using the non-steroidal aromatase inhibitor-letrozole.Seventy female Wistar rats of six weeks age were randomly divided into three groups：the control group（n=20）,PCOS model group（n=20）,metformin treatment group（n=20）.The control group were feed normally.The latter 2groups were treated by metformin and normal saline,respectively.After 14days of treatment,the rats were anesthetized and sacrificed.Endometrial condition was observed by light microscope.Using the immunohistochemical staining technique the expression level of GLUT-4and IGF-1was detected.Results The expression of glucose transporter proteins 4（GLUT-4）proteins of endometrial in PCOS rats was significantly lower（P〈0.05）than that in control group,and the expression of（IGF-1）protein in PCOS rats was higher than that in control group（P〈0.05）.The expression of GLUT-4in the treatment group increased（P〈0.05）,but was still lower than that in control group（P〉0.05）.However,compared with PCOS group,the expression of IGF-1protein in treatment group was decreased（P〈0.05）,but was still higher than that in control group（P〈0.05）.Conclusion Compared with control group,GLUT-4and IGF-1of endometrium in PCOS group were obviously different.Through the metformin treatment may reverse the situation.It is concluded that GLUT-4and IGF-1have correlation with change of PCOS endometrial.

作者吕霞

机构地区吕梁市卫生学校妇产科教研组

出处《山西医药杂志》 CAS 2014年第11期1243-1246,共4页 Shanxi Medical Journal

关键词多囊卵巢综合征二甲双胍子宫内膜胰岛素样生长因子Ⅰ 大鼠受体胰岛素 Polycystic ovary syndrome Metformin Endometrium Insulin-like growth factor-Ⅰ Rats Receptor insulin

分类号 R711.75 [医药卫生—妇产科学]

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1Ghazeeri G,Kuttek WH, Bryer-Ash M, el al. Effect of ros iglitazone on spontaneous and clomiphene citrate induced ovu lation in women polycystic ovary syndrome[J]. Fertil Steril 2003, 79 (3) : 562-566.
2李蓉,乔杰,王威,李美芝.多囊卵巢综合征患者着床窗口期子宫内膜容受性的改变[J].中国优生与遗传杂志,2007,15(10):23-25. 被引量：26
3Maliqueo M, Clementi M, Gabler F, et al. Expression of steroid receptors and proteins related to apoptosis in endome tria of women with polycystic ovary syndrome[J]. Fertil Steril,2003,80(Suppl 2) :812-819.
4Thiet MP, Osathanondh R, Yeh J. Appearance of insulin, insulin-like growth factor-1, and their receptors in the human fetalmullerian tract[J]. Am J Obstet Gyneeol, 1994, 170 (1): 152-156.
5Hardiman P, Pillay OC, Atiomo W. Polycystic ovary synd rome and endometrial carcinoma [J]. Lancet, 2003, 361 (9371): 1810-1812.
6姜艳华,陈咏健,李美芝.胰岛素受体在多囊卵巢综合征患者子宫内膜的表达与调控[J].中国药物与临床,2006,6(3):197-199. 被引量：14
7Lessey BA, Killarn AP, Metzger DA, et al. Immunohisto- chemical analysis of human uterine estrogen and progesterone receptors throughout the menstrual cycle[J]. J Clin Endocri nol Metab, 1988, 67(2): 334-340.
8Nayak NR, Giudiee LC. Comparative biology of the IGF sys- tem in endometrium,decidua, and placenta, and clinical imp lications for foetal growth and implantation disorders[J]. Pla eenta, 2003, 24(4): 281-296.
9李小永,郑桂英,张世红.多囊卵巢综合征患者胰岛素、胰岛素样生长因子及其受体的表达[J].生殖与避孕,2004,24(5):274-278. 被引量：26
10Sahin Y, Yimibes U, Kelestimur F, et al. The effects of metformin on insulin resistance, clomiphene-induced ovula tion and pregnancy rates in women with polycystic ovary syn drome[J]. Eur J Obstet Gyneeol Reprod Biol,2004,113(2) : 214-220.

二级参考文献34

1Elliott JL,Hosford SL,Demopoulos RI,et al.Endometrial adenocarcinoma and polycystic ovary syndrome: risk factors,management,and prognosis.South Med J,2001,94(5): 529-31.
2Mikola M,Hiilesmaa V,Halttunen M,et al.Obstetric outcome in women with polycystic ovarian syndrome.Hum Reprod,2001,16(2): 226-9.
3Norman RJ,Masters L,Milner CR,et al.Relative risk of conversion from normoglycaemia to impaired glucose tolerance or non-insulin dependent diabetes mellitus in polycystic ovarian syndrome.Hum Reprod,2001,16(9): 1 995-8.
4Pan HA,Wu MH,Cheng YC,et al.Quantification of Doppler signal in polycystic ovary syndrome using threedimensional power Doppler ultrasonography: a possible new marker for diagnosis.Hum Reprod,2002,17( 1 ):201-6.
5Loverro G,Vicino M,Lorusso F,et al.Polycystic ovary syndrome: relationship between insulin sensitivity,sex hormone levels and ovarian stromal blood flow.Gynecol Endocrinol,2001,15(2): 142-9.
6Juul A,Holm K,Kastrup KW,et al.Free insulin-like growth factor- Ⅰ serum levels in 1430 healthy children and adults,and its diagnostic value in patients suspected of growth hormone deficiency.J Clin Endocrinol Metab,1997,82(8):2497-502.
7Giudice LC.Insulin-like growth factor family in Graafian follicle development and function.J Soc Gynecol Investig,2001,8(1 Suppl Proceedings): S26-9.
8Guercio G,Rivarola MA,Chaler E,et al.Relationship between the growth hormone/insulin-like growth factor-I axis,insulin sensitivity,and adrenal androgens in normal prepubertal and pubertal girls.J Clin Endocrinol Metab,2003,88(3): 1 389-93.
9Diamond MP,Grainger D,Diamond MC,et al.Effects of methyltestosterone on insulin secretion and sensitivity in women.J Clin Endocrinol Metab,1998,83(12): 4 420-5.
10Unluhizarci K,Karababa Y,Bayram F,et al.The investigation of insulin resistance in patients with idiopathic hirsutism.J Clin Endocrinol Metab,2004,89(6):2 741-4.