温和灸对大鼠肛门括约肌损伤后间充质干细胞移植归巢的影响

Effect of Warm-moxibustion on Mesenchymal Stem Cells Homing of Rats with Sphincter Injury

目的:观察不同疗程温和灸对大鼠肛门括约肌损伤间充质干细胞移植后括约肌组织趋化因子SDF-1和MCP-3的表达,探讨温和灸对间充质干细胞归巢的影响。方法:将SD大鼠分为手术组、假手术组、空白对照组。每组再细分成温和灸组、干细胞移植组、温和灸联合干细胞移植组和生理盐水组。每项温和灸30 min,疗程分为24 h、3 d、7 d、14 d。治疗后取括约肌组织,运用Real-Time PCR方法检测括约肌组织趋化因子SDF-1和MCP-3的表达。结果:括约肌组织SDF-1与MCP-3表达趋势相近,SDF-1与MCP-3表达均呈现:手术组>假手术组>空白对照组,温和灸联合干细胞移植组>干细胞移植组>温和灸组>生理盐水组,且治疗后14 d>7 d>24 h>3 d。结论:温和灸可促进肛门括约肌损伤时间充质干细胞移植后的归巢,且具有一定的时效性。

Objective： To observe the warm-moxibustion on expressions of SDF-1 and MCP-3 on anal sphincter after mesenchymal stem cell （MSC） transplantation, and to explore its effects on MSC homing. Methods： SD rats were divided into operation group, sham-operation group and blank control group. Each group was subdivided into warm-moxibustion group, stem cell transplantation （SCT） group, combined warm-moxibustion and SCT group and normal saline （NS） group. Treated by warm-moxibustion fro 30 min, and the course of treatment was divided into 24 h, 3 d, 7 d, 14 d. The expressions of SDF-1 and MCP-3 were detected by Real-time PCR after treatment. Results： From the trend, the expression of SDF-1 was similar to MCP-3. The expression of them showed： operation group 〉 sham-operation group 〉 blank control group; combined warm- moxibustion and SCT group 〉 SCT group 〉 warm-moxibustion group 〉 NS group; after treatment 14 d 〉 7 d 〉 24 h 〉 3 d. Conclusion： Warm-moxibustion can promote the homing of MSC after transplantation in injured anal sphincter.