实时荧光聚合酶链反应检测支气管肺泡灌洗液结核分枝杆菌DNA诊断肺结核价值

Clinical value of real-time fluorescence polymerase chain reaction for Mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid

目的探讨实时荧光聚合酶链反应(FQ-PCR)检测支气管肺泡灌洗液结核分枝杆菌脱氧核糖核酸(TBDNA)诊断肺结核的临床价值。方法选取我院收治的115例肺结核患者作为研究对象,所有患者均进行支气管肺泡灌洗液TB-DNA检测、血清结核分枝杆菌特异性抗原(TB-SA)抗体检测、痰涂片抗酸染色镜检等。结果支气管肺泡灌洗液TB-DNA检测、血清TB-SA抗体检测、痰涂片抗酸染色镜检诊断肺结核的敏感度分别为65.2%(75/115)、50.4%(58/115)、20.9%(24/115)。支气管肺泡灌洗液检测TB-DNA诊断肺结核的敏感度显著高于血清TBSA抗体、痰涂片抗酸染色镜检(均P<0.05)。结论采用FQ-PCR检测支气管肺泡灌洗液TB-DNA诊断肺结核具有较高的敏感度。

Objective To evaluate the value of real-time fluorescence polymerase chain reaction（FQ-PCR ）for detection of Mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid （BALF）.Methods Mycobacterium tuberculosis DNA in BALF obtained from 1 1 5 patients with tuberculosis pulmonary disease were detected by FQ-PCR, Tuberculosis specific antigen（TB-SA）,and smear acid-fast respectivelly.The results were statistically analyzed.Results The sensitiveity of detection of mycobacterium was 65.2%（75/115）in FQ-PCR,50.4%（58/115）in TB-SA,20.9%（24/115）in smear acid-fast.The sensitivity of Mycobacterium tuberculos detected by FQ-PCR was higher than that by TB-SA and smear acid-fast.Conclusion The detection of TB-DNA in BALF by FQ-PCR could significantly improve the diagnosis sensitivity of pulmonary tuberculosis patients.