液态甲醛诱导小鼠肝损伤及纤维化研究

Establishing Mouse Model of Liver Fibrosis Induced by Formaldehyde

建立液态甲醛诱导小鼠肝损伤及其纤维化模型,并初步观察小鼠肝组织的病变过程.将正常昆明小鼠随机分为实验组和对照组.实验组小鼠注射15%的甲醛溶液,注射量为1 mL/kg,每周注射两次,并且每天用1%的甲醛溶液喂养.对照组小鼠以纯净水正常喂养,分别在实验开始后的第3、6、8周处死实验组小鼠各4只,肉眼及病理切片观察其肝脏形态的改变,发现小鼠的体重均呈增长趋势.与实验组小鼠比较,对照组小鼠体重增长显著(P<0.05).第8周小鼠肝脏出现明显的病变,表面粗糙,颜色暗黄,出现深色斑点.在石蜡切片中可观察到肝脏组织变得松散,窦周隙(Disse’s space)增大.肝星状细胞(hepatic stellate cell,HSC)较对照组明显增多.随着甲醛处理时间的延长,HSC不同程度地增多,肝损伤病变程度越来越严重.

To establish mouse model of hepatic fibrosis by formaldehyde, and preliminarily observe pathological state of evolution of liver fibrosis in mice. 16 male mice were randomly divided into experimental group and control group. The experimental group was given 15% formaldehyde （1mL/kg, twice per week） diluted with H2O for 8 weeks by intraperitoneal injection, and this group freely drink water with 1% formaldehyde solution every day. The matched group drank purified water. After 3 weeks, 6 weeks and 8 weeks, 4 mice were killed in each group. The pathological changes in liver were estimated through gross necropsy and microscope. Results： Weight showed a trend of growth in mice. Compared with experimental group, control group mice body weight growth trends was in significantly （ P〈0.05 ）. After 8 weeks induced by formaldehyde, mice liver appeared obvious fibrosis symptoms, the liver tissue became hard, rough in surface and dark patches; paraffin section showed that there was loose tissue and increased Disse＇ s space in fibrosis liver. Compared with control group, the number of hepatic stellate cell grows notably. With the experiment prolonged, increased in different degree HSC, hepatic fibrosis degree is more and more serious.