摘要
目的探讨鸦胆子油乳注射液对C6胶质瘤细胞增殖的影响。方法体外培养大鼠C6胶质瘤细胞,MTT法检测鸦胆子油乳注射液对C6胶质瘤细胞增殖的抑制作用;免疫细胞化学染色法和western blot法检测鸦胆子油乳注射液对C6胶质瘤细胞caspase-3蛋白表达的影响。结果 MTT法显示,鸦胆子油乳注射液对C6胶质瘤细胞增殖的抑制具有剂量依赖性。免疫细胞化学染色和western blot检测显示,随着鸦胆子油乳注射液浓度增加,caspase-3蛋白表达明显上调。结论鸦胆子油乳注射液能通过促进凋亡基因caspase-3蛋白表达的上调,从而抑制C6胶质瘤细胞增殖。
Objective To explore the effect of brucea javanica oil emulsion on proliferation of C6 glioma cells.Methods Rat C6 glioma cells were cultured in vitro.MTT colorimetric assay was used to measure the inhibitory effect of brucea javanica oil emulsion on C6 golima cells.Immunohistochemical staining and western blot was used to detect the effect of Brucea javanica oil emulsion on the caspase-3 expression of C6 glioma cells.Results MTT demonstrated that the inhibition rate of Brucea javanica oil emulsion on C6 glioma cells was does-dependent.The immunohistochemical staining and western blot results showed that the caspase-3 expression of C6 glioma cells was remarkably increased with the increase of dose of brucea javanica oil emulsion too.Conclusion Brucea javanica oil emulsion can inhibit proliferation of C6 glioma cells by increasing the expression of caspase-3 in vitro.
出处
《西部医学》
2014年第6期686-688,共3页
Medical Journal of West China
