离子交换法制备酪蛋白磷酸肽

Preparation of Casein Phosphopeptide by Ion-Exchange Resin Chromatography

分别用阴离子树脂和阳离子树脂对酪蛋白磷酸肽 (CaseinPhosphopeptide ,简称CPP)初级品N/P (摩尔比r为 7.52 )进行纯化 采用阴离子树脂纯化CPP的回收率为 :P 98.71 % ;N82 .78% ;高纯度CPP产品的r(N/P)为 5.44 对阳离子树脂纯化CPP做了初步探讨 ,研究了不同pH对纯化效果的影响 在pH 5.0的最优条件下 ,回收率P为 81 .85% ,N为 53.80 % ,r(N/P)为4.67.与阴离子树脂纯化CPP的过程相比 ,用阳离子树脂纯化CPP时 ,树脂在使用前无需转型处理 ,洗脱条件简单 ,从而大大地缩短了操作时间 。

Primary grade casein phosphopeptide (CPP) with a N/P molar ratio of 7.52 was purified by using an ion exchange resin and cation exchange resin respectively. The recovery of CPP by using an ion exchange resin was 98.71% for P and 82.78% for N and the molar ratio of N to P for the purified product was 5.44. The effect of pH on purification using cation exchange resin was investigated. The results showed that with the optimal pH 5.0, the recovery of CPP was 81.85% for P and 53.80% for N and the molar ratio of N to P was 4.67. In comparison to using anion exchange resin, the procedure of transferring type was not necessary before sample application and the eluating condintion was also simper by using cation exchange resin. A greatly shorted operation time and concentrated product could be resulted from the use of cation exchange resin.