耐高渗酵母产赤藓糖醇的影响因素

Factors Affecting Erythritol Production by An Osmophilic Yeast

球拟酵母OS 1 94是一株单产赤藓糖醇的耐高渗酵母 ,该菌株高产赤藓糖醇的最佳培养基配方为 :葡萄糖 1 0 g/dL ,酵母膏 0 .5g/dL ,尿素 0 .1 g/dL .最适培养条件是在摇瓶转速 1 50r/min的条件下于 35℃培养 4d .在上述培养条件下 ,该菌株赤藓糖醇的耗糖转化率高达 2 9.6% .磷是限制OS 1 94菌株高产赤藓糖醇的主要因素 ,当培养液中的磷质量浓度低于 31 .5mg/L时 ,赤藓糖醇的产量最高 ;随着磷质量浓度的升高 ,该菌株赤藓糖醇的产量降低 ,而酒精的产量和生物量却有明显升高 .同时 ,OS 1 94菌株还能利用果糖、蔗糖和D 甘露糖产赤藓糖醇 .

Spherical yeast OS 194 is an only erythritol production osmophilic yeast. 10 percent of glucose ,0.5 g/dL for yeast extract and 0.1 g/dL for urea were the best components of medium,which obtained high erythritol yield by OS 194.The optimum culture conditions were 35℃,150 rpm,and 4 d. In the above conditions, the maximum erythritol yield on fermented glucose was 29.6%. In this research, it was discovered that phosphorus was the main factor restricting the erythritol production. When the phosphorus content in the medium was below 31.5 mg/L, the maximum yield was obtained. With the phosphorus content increasing, the eythritol production decreased, but the production of the ethanol and the biomass increased apparently. Meanwhile, fructose,sucrose and mannose can also be utilized to produce erythritol by this strain.