TLC及HPLC测定红曲产品中的桔霉素

Analysis of Citrinin in Monascus Products by TLC and HPLC

阐述了定性及定量测定红曲产品中桔霉素含量的几种方法 .采用板层析 (TLC) ,在点样量为 1mL、点样长度 5cm条件下通过目视法 ,标准桔霉素溶液的最低检测质量浓度可达 0 .2mg/L ,板层析可作为定性检验红曲产品中桔霉素的简便方法 .根据红曲样品预处理方法及HPLC检测器的不同 ,确定了两套准确可靠的定量检测方法 .高效液相色谱结合荧光检测的最低检测限为 0 .60ng .HPLC上样液最低可检测的质量浓度为 0 .1mg/L .在 0 .1～ 1 0mg/L的质量浓度范围内 ,标准桔霉素溶液的质量浓度与峰面积的线性关系良好 ,达到R2 =0 .998.

The methods for the determination of citrinin contents in Monascus products were described in this paper. The detecting limit for the standard citrinin solution is 0.2 mg/L when spotting 1ml of standard citrinin onto TLC with the length of 1cm by observing under UV (365 nm) with naked eye. Three types of methods were employed by using different HPLC detectors and the pretreatment of the samples, and two of them were found satisfied in recovery rate, repeatability test and accuracy. The lowest detecting limit of the citrinin by HPLC with fluorescence detector is 0.6 ng. The lowest detecting concentration of the citrinin solution by using HPLC is 0.2 mg/L. The linear relationship between the contents of the standard citrinin and the peak area is satisfied with the value of R 2 being 0.998.