15%戊唑·克百威悬浮种衣剂的高效液相色谱分析

Determination of Tebuconazole+Carbofuran 15% FSC by HPLC

建立一种同时分析克百威和戊唑醇的反相高效液相色谱法。以甲醇+水为流动相,使用C18色谱柱和紫外检测器,在220 nm波长下对试样中克百威和戊唑醇进行分离和定量分析。结果表明:克百威和戊唑醇的线性相关系数分别为0.9996和0.9991,标准偏差分别为0.058和0.044,变异系数分别为0.42%和4.15%,平均回收率分别为99.94%和99.82%。方法操作简单、快速、准确,适用于15%戊唑·克百威悬浮种衣剂的定性和定量分析。

A reverse phase HPLC method was developed for quantitative determination of tebuconazole＋carbofuran 15%FSC on C18 column with UV detector at 220 nm, using methanol and water as mobile phase. The results showed that the linear correlation coefficients of carbofuran and tebuconazole were 0.999 6 and 0.999 1, the standard deviations were 0.058 and 0.044, the variation coefficients were 0.42% and 4.15%, the average recoveries were 99.94%and 99.82%, respectively. The method was simple, rapid, accurate and suitable for qualitativ and quantitative analysis of tebuconazole＋carbofuran 15%FSC.