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高效液相色谱法测定氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的含量 被引量:5

Determination of morphine,guaifenesin and glycyrrhizic acid in ammonium glycyrrhiza oral solution by HPLC
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摘要 目的:建立高效液相色谱法测定氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的含量方法。方法采用C8和C18柱,流动相为0.05 mol/L磷酸二氢钾溶液-0.0025 mol/L庚烷磺酸钠溶液-乙腈,流速为1.0 ml/min,柱温为35℃,检测波长分别220 nm和260 nm。结果吗啡、愈创甘油醚和甘草酸分别在2.0~20.2、20.3~203.0、10.0~100.0μg/ml范围内呈现良好的线性关系,平均加样回收率分别为99.4%、100.4%、101.1%。结论该方法快速、准确、重复性好,适用于氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的检测和质量控制。 Objective To establish a high performance liquid chromatography ( HPLC) method for the determination of Am-monium Glycyrrhiza Oral Solution of morphine ,guaifenesin and glycyrrhizic acid content .Methods The HPLC system consisted of C 8 and C18 column,the mobile phase was 0.05 mol/L KH2 PO4 -0.0025 mol/L Sodium heptanes-acetonitrile,the flow rate was 1.0 ml/min,the temperature of column was 35℃,the detection wavelength was set at 220 nm and 260 nm.Results Morphine,guaifenesin and glycyrrhizic acid in 2.0-20.2,20.3-203.0,10.0-100.0μg/ml range showed a good linear relationship ,the average recoveries were 99.4%,100.4%,101.1%.Conclusion This method was rapid ,accurate and suitable for quality control of morphine ,guaifenesin and glycyrrhizic acid in Ammonium Glycyrrhiza Oral Solution .
出处 《东南国防医药》 2014年第3期229-232,共4页 Military Medical Journal of Southeast China
基金 军队医疗机构制剂标准提高科研专项重点课题(13ZJ01)
关键词 氯化铵甘草 吗啡 愈创甘油醚 甘草酸 含量测定 高效液相色谱法 Ammonium Glycyrrhiza Morphine Guaifenesin Glycyrrhizic acid determination HPLC
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