高效液相色谱法测定氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的含量

Determination of morphine,guaifenesin and glycyrrhizic acid in ammonium glycyrrhiza oral solution by HPLC

目的：建立高效液相色谱法测定氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的含量方法。方法采用C8和C18柱，流动相为0．05 mol／L磷酸二氢钾溶液-0．0025 mol／L庚烷磺酸钠溶液-乙腈，流速为1．0 ml／min，柱温为35℃，检测波长分别220 nm和260 nm。结果吗啡、愈创甘油醚和甘草酸分别在2．0～20．2、20．3～203．0、10．0～100．0μg／ml范围内呈现良好的线性关系，平均加样回收率分别为99．4％、100．4％、101．1％。结论该方法快速、准确、重复性好，适用于氯化铵甘草口服溶液中吗啡、愈创甘油醚和甘草酸的检测和质量控制。

Objective To establish a high performance liquid chromatography （ HPLC） method for the determination of Am-monium Glycyrrhiza Oral Solution of morphine ,guaifenesin and glycyrrhizic acid content .Methods The HPLC system consisted of C 8 and C18 column,the mobile phase was 0.05 mol/L KH2 PO4 -0.0025 mol/L Sodium heptanes-acetonitrile,the flow rate was 1.0 ml/min,the temperature of column was 35℃,the detection wavelength was set at 220 nm and 260 nm.Results Morphine,guaifenesin and glycyrrhizic acid in 2.0-20.2,20.3-203.0,10.0-100.0μg/ml range showed a good linear relationship ,the average recoveries were 99.4%,100.4%,101.1%.Conclusion This method was rapid ,accurate and suitable for quality control of morphine ,guaifenesin and glycyrrhizic acid in Ammonium Glycyrrhiza Oral Solution .