丙戊酸钠增强免疫细胞对人膀胱癌细胞的杀伤作用

Sodium valproate enhances the cytotoxicity of immune cells to human bladder cancer cells

目的:探讨丙戊酸钠(VPA)对膀胱癌细胞MICA表达的影响以及所产生的肿瘤免疫作用,以期为防治膀胱癌复发和浸润提供新的治疗方法。方法:采用不同浓度VPA处理人膀胱尿路上皮癌T24细胞后,用半定量RT-PCR和流式细胞术检测癌细胞中MICA mRNA和蛋白表达。用乳酸脱氢酶法检测外周血单个核细胞(PBMCs)对经VPA处理的T24细胞的杀伤作用。结果:VPA从mRNA和蛋白水平诱导T24细胞表达MICA,并增强T24细胞对PBMCs细胞毒作用的敏感性。结论:VPA能增强膀胱癌对免疫细胞杀伤作用的敏感性,可作为防治膀胱癌的辅助药物。

Objective.. To investigate the effect of sodium valproate （VPA） on the expression of MICA and PB- MCs-mediated cytotoxicity in T24 bladder cancer cells so as to provide a new method for treatment and prevention of recurrent and infiltrating bladder cancer. Method：T24 cancer cells were treated with VPA and the expression of MICA at the mRNA and protein levels were analyzed by RT-PCR and flow cytometry. Cytotoxicity of PBMCs a- gainst the T24 cells was investigated with the lactate dehydrogenase release assay. Result： VPA increased MICA expression in T24 cells and sensitizes cancer cells to cytotoxicity of PBMCs. Conclusion：VPA sensitizes human blad- der cancer cells to cytotoxicity of immune cells.