电毁损锥体束与内囊出血法建立痉挛性脑瘫大鼠模型的比较

Comparison of electronic lesion of pyramidal tract and internal capsule hemorrhage in establishing spastic rat models

目的比较电毁损锥体束与内囊出血2种方法建立痉挛性脑瘫大鼠模型的差异。方法84只SD大鼠按随机数字表法分为电毁损模型组、内囊出血模型组和对照组，每组28只，每组再分为4个不同的观察时间亚组（术后7d、14d、21d及28d组），每组7只。记录每组动物一般状况及行为学评分．检测腓肠肌Hoffman反射，采用骨骼肌肌球蛋白ATP酶染色计算股四头肌Ⅰ型肌纤维比例。结果与对照组比较，电毁损模型组及内囊出血模型组大鼠术后各项神经功能缺损症状持续时间更长，腓肠肌Hoffman反射波幅增高且潜伏期缩短更明显，差异有统计学意义（P〈0．05），并分别持续至术后28d和14d。电毁损模型组及内囊出血模型组大鼠的股四头肌Ⅰ型肌纤维比例较对照组大鼠明显增高，差异有统计学意义（P〈0．05），并分别持续至术后21d和14d。其中电毁损模型组大鼠的行为学评分、腓肠肌Hoffman反射的潜伏期、波峰值和股四头肌Ⅰ型肌纤维比例异常，与内囊出血模型组大鼠相比更高，差异有统计学意义（P〈0．05）。结论电毁损模型较内囊出血模型肌张力增高更加显著，持续时间更长。

Objective To compare the differences between electronic lesion of pyramidal tract and internal capsule hemorrhage in establishing spastic rat models. Methods Experimental animals were randomly assigned into three groups： electronic lesion group （EL）, internal capsule hemorrhage group （ICH） and control group （CON, n=28）. Seven, 14, 21 and 28 d after each treatment, the general conditions of the rats and behavioral tests on them were recorded; and the gastrocnemius Hoffrnan reflex （H reflex） was detected; and the percentage of type I muscle fiber in quadriceps femoris were examined by skeletal muscle myosin ATP staining. Results Rats in EL group and ICH group showed significant differences in behavioral tests as compared with CON group （P〈0.05）; the duration of symptoms of neurological deficit was significantly longer in EL group than the other two groups （P〈0.05）. In contrast to CON group, the amplitude of gastrocnemius H reflex in EL group and ICH group was increased and the latency of H reflex was decreased with significant difference （P〈0.05）, which could be observed till 28 d and 14 d, respectively, after each treatment. The percentage of type I muscle fiber in quadriceps femoris EL and ICH groups was obviously increased as compared with that in CON group （P〈0.05）, which persisted to 21 and 14 d, respectively, after each treatment. Meanwhile, rats in EL group showed significant differences in behavioral test, gastrocnemius H reflex and percentage of type I muscle fiber in quadriceps femoris as compared with ICH rats （P〈0.05）. Conclusion The muscle tone of EL rats is higher, and lasts longer than that oflCH rats.