介入热化疗栓塞对肝癌患者血清细胞因子水平变化的临床研究

Changes of serum cytokine levels after interventional hyperthermia chemoembolization for patients with liver cancer

目的探讨介入热化疗栓塞对肝癌患者血清细胞因子的影响及疗效观察。方法采用ELISA法检测60例原发性肝癌患者(介入热疗组30例,常规介入组30例)治疗前后血清8种细胞因子的变化,并对两组数据进行对比分析。结果治疗前血清8种细胞因子(IL-17、IL-10、IFN-γ、IL-8、IL-1β、IL-6、IL-1α、TNF-α)在两组间差异无统计学意义(P>0.05)。治疗后1月介入热疗组IL-17水平降低伴有IL-10水平升高,而常规介入组未出现明显变化,血清IL-17和IL-10在两组间差异有统计学意义(P<0.05)。介入热疗组2年生存率明显高于常规介入组(73.3%vs 56.7%,P<0.05)。结论介入热化疗栓塞能使血清IL-17水平降低、IL-10水平升高,能抑制肿瘤生长,提高免疫力,延长生存时间,是治疗中晚期肝癌的一种安全有效的方法。

Objective To investigate the changes of serum cytokine levels after interventional hyperthermia chemoembolization（IHCE） in patients with liver cancer. Methods Sixty patients with primary liver cancer were enrolled in the study, including 30 cases undergoing interventional hyperthermia chemoembolization （IHCE group） and 30 cases receiving conventional interventional chemoembolization （ICE group）. The levels of serum IL-17 ,IL-10, IFN- γ,IL-8, IL-1β,IL-6,IL-1α and TNF-α were measured by ELISA and the clinical outcome was documented. Results Serum cytokine levels were of no difference between two groups before treatment （P 〉 0.05 ） , while there were significant differences in IL- 17 and IL-10 levels after treatment between two groups （P 〈 0.05）. After treatment,IL-17 level was decreased and IL-10 level was increased in IHCE group （P 〈 0.05 ）, while no changes were observed in ICE group. Two-year survival rate in IHCE group was higher than that in ICE group （73.3% vs 56.7% ,P 〈 0.05）. Conclusion Interventional hyperthermia chemoembolization has better efficacy than conventional interventional therapy for advanced liver cancer patients. The favorable outcomes are associated with decreased serum IL-17 and increased IL-10 levels.