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系统性红斑狼疮患者外周血B细胞中狼疮活动相关微小RNA的表达 被引量：4

Differential expression analysis of the disease activity related miRNAs in peripheral blood B lymphocytes of systemic lupus erythematosus patients
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摘要 目的 探讨SLE患者外周血B细胞中SLE活动相关的微小RNA（miRNA）表达与SLE活动时SLEDAI评分、补体C3水平的关系.方法 在芯片分析技术筛选出SLE患者外周血B细胞中SLE活动相关miRNA的基础上,利用茎环状引物实时定量-PCR技术在16例SLE重度活动期和16例稳定期患者外周血B细胞中进行验证.采用t检验和直线相关分析进行统计学分析.结果 茎环状引物实时定量-PCR技术验证发现SLE重度活动期5个miRNA（miR-191,miR-103,miR-17,miR-1224-3P,miR-1249（0.50±0.21,0.51±0.27,0.8±0.6,1.6±1.4,2.2±2.0）较稳定期（0.68±0.24,0.80±0.39,1.7±1.0,3.5±2.6,4.6±2.9）下调（t=-2.15,t=-2.26,t=-2.72,t=-2.45,t=-2.58,P均＜0.05）;miR-155（0.59±0.25）较稳定期（0.38±0.21）上调（t=2.36,P＜0.05）.同时血清补体C3下降程度与miR-191表达呈正相关（r=0.625,P＜0.05）,与miR-155表达呈负相关（r=-0.548,P＜0.05）.结论 SLE活动期与静止期患者外周血B细胞中存在着miRNA差异表达,且其中miR-191、miR-155与补体C3下降有关,提示miRNA可能参与SLE患者狼疮活动过程. Objective To investigate the relationship between the disease activity related miRNAs in the peripheral blood B lymphocytes and SLEDAI score and Serum complement C3 level in systemic lupus erythmatosus （SLE） patients.Methods After identifying the miRNAs related to lupus activity by miRNA microarray chip analysis,Stem-loop real-time polymerase chain reaction （PCR） technology was used to confirm the related miRNAs in 16 active and 16 stable SLE patients.T-test and liner correlation correlation analysis were used for statistical analysis.Results Five down regulated miRNAs （miR-191,miR-103,miR-17,miR-1224-3P,miR-1249,0.50±0.21 vs 0.68±0.24,0.51±0.27 vs 0.80±0.39,0.8±0.6 vs 1.7±1.0,1.6±1.4 vs 3.5±2.6,2.2±2.0 vs 4.6±2.9; t=-2.15,t=-2.26,t=-2.72,t=-2.45,t=-2.58,P＜0.05） and one upregulated miRNA （miR-155,0.59±0.25 vs 0.38±0.21; t=2.36,P＜0.05） were found in severe active SLE patients by real-time PCR.The expression level of miR-191 was significantly positively correlated with serum C3 level （r=0.625,P＜0.05）,while miR-155 was significantly negatively correlated with serum C3 level （r=-0.548,P＜0.05）.Conclusion miRNAs are differentially expressed in peripheral blood B lymphocytes in severe active and stable SLE patients.miR-191 and miR-155 are significantly related to serum C3 levels,which indicate that miRNAs may play important roles in the status of lupus disease activity.
作者 陈芳 陈朝生 章慧娣 薛向阳 丁晓凯 孙莉 陈丹 黄朝兴
机构地区 温州医学院附属第一医院肾内科 温州医学院附属第一医院风湿科 温州医学院免疫微生物学教研室
出处 《中华风湿病学杂志》 CAS CSCD 北大核心 2014年第6期411-413,共3页 Chinese Journal of Rheumatology
基金 浙江省温州市科技计划（Y20100198）
关键词 红斑狼疮 系统性 微小RNAS B淋巴细胞 Lupus erythmatosus, systemic Micro RNAs B-lymphocytes
分类号 R593.241 [医药卫生—内科学]
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参考文献14

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共引文献9

同被引文献51

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中华风湿病学杂志
2014年 第6期

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