整合素a5b1对胃癌细胞黏附、转移及耐药的影响

Role of integrin a5b1 in adhesion, migration and extramedullary infiltration of gastric cancer cells

目的:探讨整合素a5b1 mRNA表达水平对胃癌细胞黏附、迁移、髓外浸润过程的影响.方法:采用Western blot检测法及RT-PCR分别测量HTB-103、CRL-5822、CRL-5971及CRL-5973等不同对数生长期的白细胞株整合素a5b1蛋白表达水平及整合素a5b1 mRNA水平.将不同胃癌细胞株随机分为实验组及对照组,实验组加入整合素a5b1抗体,对照组加入同型IgG,观察两组胃癌细胞与ECV304细胞系的黏附率、细胞迁移率及白细胞穿过人工基质膜的浸润能力.结果:CRL-5822、CRL-5971及CRL-5973细胞整合素a5b1 mRNA相对表达量分别为(0.0821±0.0128)、(0.185±0.0082)、(0.798±0.042)明显高于HTB-103细胞(0.0002±0.0000)(P<0.05).观察组CRL-5822、CRL-5971及CRL-5973细胞株黏附率(52.16%±2.52%、44.22%±2.59%、33.58%±4.06%)、迁移率(45.96%±1.21%、38.86%±1.99%、24.65%±3.28%)及浸润率(29.85%±4.63%、21.31%±3.24%、13.67%±3.48%)、显著低于对照组黏附率(83.89%±7.21%、71.17%±7.38%、60.89%±10.59%)、迁移率(75.41%±9.51%65.78%±14.62%,49.91%±13.47%)及浸润率(42.63%±7.69%,29.92%±5.47%,21.59%±6.72%)(P<0.05),而两组HTB-103细胞黏附率、迁移率及浸润率无统计学差异(P>0.05).结论:黏附因子整合素a5b1可能参与胃癌细胞的形成、聚集、生长及浸润的过程,其表达水平与胃癌病程进展具有密切的关系.

AIM： To investigate the role of the adhesion molecule integrin a5bl in adhesion, migration and extramedullary infiltration of gastric cancer cells.METHODS： Immunohistochemistry and RT- PCR were used to measure integrin a5blprotein and mRNA expression levels in cell lines HTB-103, CRL-5822, CRL-5971 and CRL-5973. Different cell lines were randomly divided into an experimental group and a control group. The experimental group was incubated with anti-in- tegrin a5bl antibody, and the control group was incubated with control IgG. After incubation, the adhesion, migration and extramedullary infiltration of the above cells and ECV304 cells were assessed. RESULTS： The relative mRNA expression levels of integrin a5bl were significantly higher in CRL-5822, CRL-5971 and CRL-5973 cells than in HTB-103 cells （0.0821 ± 0.0128, 0.185 ± 0.0082, 0.798 ± 0.042 vs 0.0002 ± 0.0000, P 〈 0.05）. The rates of adhesion, migration and extramedul- lary infiltration in CRL-5822, CRL-5971 and CRL-5973 cells in the experimental groups were significantly lower than those in the control groups （adhesion （%）： 52.16 ± 2.52 vs 83.89± 7.21, 44.22 ± 2.59 vs 71.17 ± 7.38, 33.58 ±4.06 vs 60.89 ± 10.59; migration （%）： 45.96 ± 1.21 vs 75.41± 9.51, 38.86 ± 1.99 vs 65.78 ± 14.62, 24.65 ± 3.28 vs 49.91 ± 13.47; infiltration rate （%）： 29.85 ± 4.63 vs 42.63 ± 7.69, 21.31± 3.24 vs 29.92 ± 5.47, 13.67 ± 3.48 vs 21.59 ± 6.72; P 〈 0.05 for all）, but the above indexes did not differ in HTB-103 cells between the two groups （P 〉 0：05 for all）. CONCLUSION： The adhesion molecule integrin a5bl may be involved in the adhesion, migration and extramedullary infiltration of gastric cancer cells.