摘要
目的:研究CD133^+CXCR4^+胃癌细胞侵袭能力.方法:Transwell检测NCD133^-CXCR4^-、CD133^-CXCR4^+、CD133^+CXCR4^-、CD133^+CXCR4^+四组细胞迁移和侵袭能力.半定量酶链聚合反应检测CD133^+CXCR4^+组与CD133^+CXCR4^-组细胞上皮间质转化(epithelial mesenchymal transition,EMT1相关因子表达。分别用基质细胞衍生因子-1α(slromal cell derived factor-1α,SDF-1α)、AMD3100作用KATO-Ⅲ细胞后,Transwell检测CD133+ CXCR4^+组与CD133^+CXCR4^-组细胞侵袭能力,半定量酶链聚合反应检测KATO-Ⅲ细胞中EMT相关因子表达.结果:CD133^+CXCR4^+组平均迁移细胞数(136.67±14.36)高于CD133^+CXCR4^-组(55.33±7.37.P=0.01).CD133^+CXCR4^+组E-cadherin mRNA相对灰度值(0.3068±0.03991低于CD133^+CXCR4^-组(0.7665±0.0899,P=0.005).CD133^+CXCR4^+组N—Cadherin mRNA表达(0.5852±0.0453)、Snail mRNA表达(0.9178±0.0788)均高于CD133^+CXCR4^-组(0.2980±0.0626,P=0.006;0.6468±0.1506.P=0.03).CD133^+CxCR4^+组中,与对照组相比,SDF-1α组侵袭细胞数明显上升(P=0.033).SDF-1α组E-cadherin mRNA表达下降(P=0.018),而AMD3100组E-cadherin mRNA表达上升(P=0.008).SDF-1α SRSnail mRNA表达上升(P=0.028),而AMD3100组Snail mRNA表达下降(P=0.006).然而CD133^+CXCR4^-组中.SDF-1α组侵袭细胞数无明显变化.SDF-1α与AMD3100组E—cadherin mRNA与Snail mRNA表达均无明显变化.结论:CD133^+CXCR4^+胃癌细胞可能通过EMT和SDF-1α/CXCR4获得高侵袭能力.
AIM: To assess the invasive capacity of CD133^+ CXCR4^+ gastric cancer cells. METHODS: The invasive and migration capacities of CD133^-CXCR4^-, CD133^-CXCR4^+, CD133^+CXCR4^-, and CD133^+CXCR4^+ gastric cancer ceils were assessed by Transwell assays. The mRNA expression of EMT related factors in CD133^+CXCR4^- and CD133^+CXCR4^+ cells was detected by semi-quantitative RT- PCR. After SDF-1α and AMD3100 were used to stimulate and inhibit KATO-Ⅲ cells, respectively, the invasive ability of CD133^+CXCR4^+ and CD133^+CXCR4^- cells was assessed, and themRNA expression of EMT related factors was detected by semi-quantitative RT-PCR. RESULTS: The mean number of migrated cells in the CD133^+CXCR4^- group was significantly more than that in the CD133^+CXCR4^- group (136.67± 14.36 vs 55.33 ± 7.37, P = 0.01). The expressive level of E-cadherin mRNA in the CD133^+CXCR4^+ group was significantly lower than that in the CD133^+CXCR4^- (0.3068 ± 0.0399 vs 0.7665 ± 0.0899, P = 0.005). The expressive levels of N-cadherin and Snail mRNAs in CD133^+CXCR4^+ cells were significantly stronger than those in CD133^+CXCR4^- cells (0.5852 ± 0.0453 vs 0.2980 ±0.0626, P = 0.006; 0.9178 ± 0.0788 vs 0.6468 ± 0.1506, P = 0.03). In CD133^+CXCR4^+ cells, SDF-1α treatment significantly increased the number of invasive cells (P = 0.033), decreased the expressive level of E-cadherin mRNA (P = 0.018), and increased the expressive level of Snail mRNA (P = 0.028). AMD3100 treatment significantly increased E-cadherin mRNA expression (P = 0.008) and decreased Snail mRNA expression (P = 0.006). In CD133^+CXCR4^- cells, SDF-1α or AMD3100 treatment did not significantly alter the invasion and expressive levels of E-cadherin and Snail mRNAs. CONCLUSION: The high invasion ability of CD133^+CXCR4^+ gastric cancer cells may be mediated by EMT and SDF-1α/CXCR4.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第14期1977-1983,共7页
World Chinese Journal of Digestology
基金
蚌埠医学院研究生创新计划基金资助项目,No.Byycx1323
国家自然科学基金资助项目,No.81101850~~