不同载荷运动对去势雌性大鼠骨关节软骨形态学及代谢的影响

Effect of different loading exercise on morphology and metabolism of articular cartilage in ovariectomized female rats

目的研究不同载荷情况下运动对去势大鼠骨关节软骨形态学和代谢方面的影响。方法 SD大鼠在成功制备为骨质疏松模型后,随机分为安静组、低载荷运动组、中载荷运动组和高载荷运动组,各10只进行跑台运动;运动3个月后处死大鼠,收取膝关节标本及血液,进行大鼠血清相关物质的检测,进行关节软骨形态学(HE染色、番红O染色、甲苯胺染色)检查及免疫组化检测Ⅱ型胶原蛋白含量。结果形态学及血清检测结果表明,中载荷运动组有利于维持关节软骨的稳态(P<0.05);而与安静组、低载荷和中载荷运动组相比较,高载荷运动组则不利于关节软骨的生长,使关节软骨出现退变样改变(P<0.05)。结论不同载荷运动对大鼠膝关节软骨的影响不同,适度载荷运动对预防和治疗骨质疏松有较好的临床指导意义。

Objective To investigate the influence of loading exercise on morphology and metabolism of articular cartilage in ovariectomized rats. Methods Forty female Sprague-Dawley rats were randomly assigned into 4 groups ： ovariectomized （ OVX）, medium loading exercise ovariectomized plus low loading exercise （low loading OVX-RUN） , ovariectomized plus （medium OVX-RUN）, ovariectomized plus high loading exercise （high loading OVX- RUN） ; After 12 weeks, the following variables were compared among the 4groups. Knee joint and blood samples were collected; Test of rat serum related material were carried on the articular cartilage morphology （HE dyeing, sarranine O dyeing, toluidine） examination and immunohistochemical staining to detect the content of type Ⅱ collagen in articular cartilage. Results Medium OVX-RUN group was helpful to maintain the steady state of articular cartilage （P 〈 0. 05）. Compared with OVX, low loading OVX-RUN group and medium OVX-RUN group, high loading OVX-RUN group was not conducive to the growth of the articular cartilage,induce the degenerative-like changes in articular cartilage （P 〈 0. 05）. Conclusions The different loading exercise has different effect on the cartilage of rats, moderate loading exercise on the prevention and treatment of osteoporosis has useful clinical significance.