结缔组织生长因子通过增强Caspase-3活性调节ox-LDL诱导THP-1细胞凋亡的机制

Mechanism of connective tissue growth factor enhance Caspase-3 activity to regulate ox-LDL induced THP-1 apoptosis

目的:研究动脉粥样硬化过程中结缔组织生长因子(CTGF)在巨噬细胞凋亡过程中发挥的作用及机制。方法:对人巨噬细胞(THP-1)分组进行干预,采用SiRNA基因沉默方法,对CTGF基因进行靶向沉默,用细胞流式、实时定量、蛋白印迹及酶活性检测等技术,阐明CTGF在氧化性低密度脂蛋白(ox-LDL)诱导THP-1凋亡过程中起的作用及机制。结果:CTGF在ox-LDL刺激THP-1细胞组高表达,而CTGF靶向性SiRNA可以降低其表达;沉默CTGF后,THP-1凋亡率明显降低,并且与Caspase-3酶活性呈正相关。结论:内源性的CTGF可以通过增强Capsase-3酶活性来促进THP-1的凋亡。

Objective：To research the role and mechanism of connective tissue growth factor （CTGF） during process of the macrophage apoptosis. Method.. We stimulated the different group of the human macrophage （THP- 1）. We used the RNA interfering technology to influence gene of CTGF, and observed the role and mechanism of CTGF during process of the THP-1 apoptosis, which were detected by flow cytometry, PCR, Western blot, and ELISA. Resuit..Ox-LDL induced the high expression of CTGF in THP-1 ceils, and the SiRNA of CTGF decreased the expression of CTGF, this effect also influenced the apoptosis rate of THP-1 cells. In addition, the activation of Caspase-3 also changed with the SiRNA of CTGF. Conclusion： Endogenous CTGF can enhance the activity of Caspase-3 to induce the THP-1 cells apoptosis.