芪苈强心胶囊对阿霉素所致心力衰竭大鼠氧化损伤的影响

Experimental study of Qiliqiangxin capsule against adriamycin induced oxidative damage in rats with heart failure

目的观察芪苈强心胶囊对慢性心力衰竭(CHF)模型大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平的影响。方法 Wistar大鼠40只,随机抽取8只作为健康对照组,其余大鼠用多次间断腹腔注射阿霉素(ADR)的方法来建立大鼠CHF模型。造模成功后,随机分为模型组和芪苈强心胶囊组(0.6 g·kg^(-1)·d^(-1)),每组15只,健康对照组和模型组灌胃等容积的蒸馏水。4周后,各组大鼠称质量,取心脏称质量,计算心脏指数,腹主动脉取血测MDA、SOD水平及血清脑钠肽(BNP)水平。结果与健康对照组比较,模型组大鼠心脏指数[(3.76±0.22)g/kg vs.(2.69±0.25)g/kg]、BNP[(657±59)pg/ml vs.(203±31)pg/ml]水平均明显升高(P<0.05),而芪苈强心胶囊组分别为(3.18±0.15)g/kg和(554±43)pg/ml,均明显低于模型组,差异有统计学意义(P<O.05)。与健康对照组比较,模型组血清SOD水平明显下降[(113.33±10.78)U/ml vs.(197.67±12.93)U/ml],MDA水平明显升高[(18.72±1.79)nmol/ml vs.(7.73±1.53)nmol/ml],差异有统计学意义(P<0.05),而芪苈强心胶囊组SOD水平(151.17±12.19)U/ml明显高于模型组,MDA水平(14.61±1.27)nmol/ml明显低于模型组,差异均有统计学意义(P<0.05)。结论芪苈强心胶囊对慢性心力衰竭大鼠氧化损伤有治疗作用,其作用机制可能与清除氧自由基,抗脂质过氧化有关。

Objective To observe Qiliqiangxin capsule on chronic heart failure rat model serum level of superoxide dismutase （SOD）, malondialdehyde （MDA）. Methods Forty Wistar rats were randomly selected 8 as healthy control group, the other rats by using several intermittent intraperitoneal injection of aclriamyein （ADR） method to establish a rat model of CHF. After the success of modeling, they were randomly divided into model group and Qiliqiangxin capsule group （0. 6 g ~ kg-1 ~ d -l ） with 15 rats in each group, health control group and model group were administered with iso-volume of distilled water. After 4 weeks, the rats weight and heart mass were recorded, cardiac index, abdominal aortic blood MDA, SOD levels and serum brain natriuretic peptide levels of BNP were measured and recorded. Results Compared to the control group, cardiac index of the rats in the model group [ （3.76 ± 0.22） g/kg vs. （2.69 ± 0.25） g/kg], BNP [ （657 ±59） pg/ml vs. （203 ± 31 ） pg/ml ] levels were significantly elevated （ P 〈 0.05）, and Qiliqiangxin capsule group were （3.18 ± 0.15） g/kg and （554 ± 43） pg/ml, were significantly lower than those in the model group, the difference was statistically significant （ P 〈 0.05 ）. Compared with the healthy control group, levels of serum SOD model group decreased significantly [ （ 113.33 ± 10.78） U/ml vs. （ 197.67 ± 12.93 ） U/roll, MDA levels were significantly higher [ （ 18.72 ± 1.79 ） nmol/ ml vs. （7.73 ±1.53 ） nmol/ml ], the difference was statistically significant （ P 〈 0.05 ） and Qiliqiangxin capsule group SOD （ 151.17 ~ 12.19） U/ml was significantly higher than the model group, the levels of MDA （ 14.61 -＋ 1.27） nmol/ml was significantly lower than that in the model group, the differences were statistically significant （ P 〈 0.05）. Conclusion Qiliqiangxin capsule has a therapeutic effect on chronic heart failure rats. The mechanism may be related to scavenging oxygen free radicals and inhibiting lipid per oxidation.