血红素蛋白二聚、寡聚与多聚

Dimerization,Oligomerization and Polymerization of Heme Proteins

血红素蛋白在生物体系中执行多种重要的生物功能,如氧贮存和运输、电子传递、生物催化和生物传感等。尽管大多数血红素蛋白是以单体形式存在,一些血红素蛋白在体外和/或体内,还会形成同源聚合物。本文综述了血红素蛋白的二聚、寡聚与多聚研究进展,包括肌红蛋白、细胞色素c、胞红蛋白,细胞色素b5、细胞色素b562、亚硝酸盐还原酶、血红素传感蛋白和血红素转运蛋白等,着重介绍其结构与功能以及多聚体分子设计的方法与应用。这些研究进展,一方面增强了我们对生物体系中血红素蛋白结构与功能关系的认识,另一方面也赋予了我们通过多聚方法设计功能蛋白来调控和利用血红素蛋白的能力。

Heme proteins play various important roles in biological systems such as oxygen storage and transport, electron transfer, catalysis and signaling. Although most heme proteins exist as a monomer, homomeric complexes are also observed for some heine proteins in vitro and/or in vivo. This review summarized the progress of dimerization, oligomerization and polymerization of heme proteins, including myoglobin, cytochrome c, cytoglobin, cytochrome b5, cytochrome b562, nitrite reductase, heine-based sensor and heme transport proteins, etc. , and focused on the resultant structures and functions, as well as the approaches for rational design of polymers and their applications. These progresses, on one hand, enhanced our knowledge of the structure-function relationship of heine proteins in biological systems, and on the other hand, endowed us an ability of regulating and utilizing heine proteins by functional protein design through polymerization.