仪器分析法与显微镜法检测尿液细胞成分比较分析

A comparative analysis between instrument methods and microscope test method in testing urine red blood cells and urine white blood cells

目的探讨仪器分析法与显微镜法检测尿液细胞成分的异同,为提高尿液检测质量提供依据。方法采用UF-100全自动尿沉渣分析仪、MA-4280KB尿干化学分析仪和OlympusCX21显微镜3种方法对710份尿样进行检测,并对结果进行比较分析。结果尿沉渣分析仪、尿干化学分析仪与显微镜法检测尿液红细胞(RBC)和白细胞(WBC)结果差异均有统计学意义(均P<0.01),尿沉渣分析法与尿干化学分析法差异亦有统计学意义(WBC:P<0.01,RBC:P<0.05),尿沉渣分析法与尿干化学分析法联合检测尿液白细胞和红细胞,并联时灵敏度有一定提高,串联时特异度提高明显。结论 3种方法检测原理不同导致检验结果存在差异,要根据临床诊断目的选择合适的检测方法,必要时可联合应用,以提高检验结果的准确性。

Objective To explore the similarities and differences between the instrument analysis detection method and microscopy in urine cell components,to provide a basis for improving the quality of urine test.Methods We used UF-1 00 automatic urine sediment analyzer,MA-4280KB urine dry chemistry analyzer and OlympusCX21 microscopy to test 71 0 samples,and the results were compared. Results Urine analyzer,urine dry chemistry analyzer and microscopy detection of urinary red blood cell （RBC）and white blood cell （WBC）showed statistically significant differences （P〈0.01 ）.Urinary sediment analysis and urine dry chemistry analysis had significant difference （WBC：P〈0.01 ,RBC：P〈0.05 ）.When urinary sediment analysis was used with urine dry chemistry analysis to test WBC and RBC in urine,the sensitivity was improved in parallel,and the specificity was improved in series.Conclusions Different detection principles of the three methods lead to differences in test results.Appropriate detection method should be chosen according to the objective of clinical diagnosis,and combination of detection method can be used,when necessary,to improve the accuracy of test results.