巴中产栀子对大鼠利胆退黄药理效应研究

Research on Pharmacological Effect of Fructus Gardeniae from Bazhong Preventing Jaundice of Rats

目的:研究巴中产栀子预防黄疸产生的效果。方法:选取正常大鼠,分别灌胃给予巴中、湖北、江西和江津产栀子水溶性有效部位,70%醇溶性有效部位;化学氧化法测定大鼠血清中总胆红素TBIL,酶法测定大鼠血清中总胆固醇CHOL和甘油三酯TG,考察不同产地栀子对正常大鼠血清中TBIL、TG和CHOL的影响。结果:与空白对照组比较,4个产地栀子水溶性有效部位或70%醇溶性有效部位均具有明显降低正常大鼠TBIL、TG和CHOL作用,巴中产栀子提取物利胆退黄的效果较其它三产地好,特别是水溶性有效部位,极显著优于醇溶性有效部位(P<0.001)。巴中产栀子水溶性有效部位极显著优于江西产栀子水溶性有效部位(P<0.001);非常显著优于湖北产栀子水溶性有效部位(P<0.01)。结论:巴中产栀子品质更优,预防黄疸产生的效果更佳,药理研究和开发应用值得广泛关注。

Objective： To study the effect of fructus gardeniae from Bazhong preventing jaundice. Methods ： The normal rats were selected and administrated water extraction and 70% ethanol extraction of fruetus gardeniae orally. Chemical oxidation method was used to measure the serum total hilirubin TBIL in rats. The enzyme method was used to measure the total cholesterol CHOL and triglyceride TG in rabbit bile. The levels of TBIL, TG and CHOL in normal rats＇ blood serum were determind by administrating gardenia grown in different regions. Results ： Compared with normal blank control group, gardenias in four areas water soluble parts, or 70% ethanol soluble parts obviously reduced CHOL, TG and CHOL of normal rats. Fructus gardeniae extract from Bazhong had better effect than other three regions＇. What＇s more, water soluble effective components had better effect than alcohol soluble parts （ P〈0.001 ）. The water soluble effective components of gardenia from Bazhong had more significant effect than Jiangxi＇s （ P〈0.001 ）. And it had the most significant effect than water soluble effective components of fructus gardeniae from Hubei （ P〈0.01 ）. Conclusion ： Fructus gardeniae from Bazhong has better quality and better effect on preventing jaundice than other that from three areas. The research and development about fructus gardeniae from Bazhong is worthy of being focused on.