UPLC法同时测定复方守宫散中蒽醌类含量

Simultaneous Determination of Anthraquinone Contents in Compound Shougong Powder by UPLC

目的：建立UPLC法同时测定复方守宫散中大黄素、大黄素甲醚含量的方法。方法：用Acquity BEH C18（2.1 mm×100 mm,1.7μm）,甲醇-0.1%磷酸水溶液（80∶20）为流动相,流速：0.25 mL/min,柱温：26℃,检测波长：254 nm。结果：大黄素、大黄素甲醚的进样量分别在0.0398~0.6625μg（r=0.9996）、0.0480~0.8000μg（r=0.9994）范围内与其峰面积呈良好的线性关系;平均回收率分别为97.07%、98.24%,RSD分别为2.26%、3.27%。结论：该方法快速、可靠、准确,可作为复方守宫散的质量控制方法。

Objective ： To establish a UPLC method for the determination of Emodin and Physcion in Compound Shougong Powder. Methods： The determination was performed on Acquity BEH Cla （ 2.1 mm×100 mm,1.7μm ） with mobile phase consisted of Methabol-0.1% phosphoric acid aqueous solution （ 80： 20 ） at a flow rate of 0.25 mL/min. Column temperature was 26℃ . The UV detection wavelength was set at 254 nm. Result ： The calibration curves were linear in the range of 0.0398-0.6625 Ix g （ r=0.9996 ） for Emodin,0.0480-0.8000μg （ r=0.9994 ）for Physcion. The average recovery was 97.07%, 98.24% and RSD was 2.26%, 3.27% respectively. Conclusion ： The determination method is fast, reliable, accurate, and it can be used for quality control of Compound Shougong Powder.