氨基胍对脊髓损伤大鼠后肢运动功能的影响

Effect of aminoguanidine on hindlimb motor function of rat with spinal cord injury

目的研究大鼠脊髓损伤后氨基胍对其后肢运动功能的影响。方法选择62只体质量、活动力相似的大鼠制作大鼠脊髓压迫伤模型,根据随机数字表法分为观察组(氨基胍治疗组)31只及对照组(未治疗组)31只,比较2组大鼠后肢运动功能。结果观察组建模后24h一氧化氮含量平均(0.851±0.0473)μmol/g·pro,一氧化氮合酶活性平均(0.269±0.0147)U/mg·pro,均明显低于对照组;建模后4周振幅测量平均(1.98±0.46)mV,潜伏期平均(8.91±0.88)ms,均显著低于对照组;BBB行为学评分(16.9±5.1)分,显著高于对照组,P均<0.05。结论氨基胍应用于脊髓损伤后大鼠能够显著改善其运动功能,可能与其抑制脊髓损伤后细胞凋亡有关。

Objective To analyze the effect of aminoguanidine on hindlimb motor function of rat with spinal cord injury.Methods We selected 62 rats whose weight and active force production were similar and made them into rat models of spinal cord compression injury.They were randomly divided into observation group （aminoguanidine group ,n=31） and control group （without treatment group ,n= 31） ,we compared the hindlimb motor function between two groups.Results In observation group ,the average nitrogen oxide was （0.851 ± 0.0473）μmol/g · Pro after 24 hours of modeling ,the average nitric oxide syn-thase activity value was （0.269 ± 0.0147） U/mg · Pro ,they were significantly lower than those of control group.After modeling for 4 weeks ,the average amplitude of Motion Evoked Potential was （1.98 ± 0.46） mV ,the average incubation period was （8.91 ± 0.88）ms ,they were significantly lower than those of control group ;BBB rating was 16.9 ± 5.1 ,it was significantly higher than that of the control group.These differences were significant ,P〈0.05.Conclusion Aminoguanidine can obviously improve the motor function of rats with spinal cord injury ;the mechanism may be to inhibit cell apoptosis after spinal cord injury.