高温胁迫下毛竹叶片色素含量与反射光谱的相关性

The Correlation between the Pigment Content and Reflectance Spectrum in Phyllostachys edulis Leaves Subjected to High Temperature

采用25℃(对照)、35℃、40℃和45℃4个温度对3年生盆栽毛竹实生苗进行处理,测定毛竹叶片色素含量和反射光谱;同时对叶片光谱反射率、一阶导数及特征参数与色素含量进行相关分析。结果表明:随着高温胁迫温度的升高,叶绿素a、叶绿素b和类胡萝卜素含量呈降低趋势;反射光谱参数SR705,PSSRa,PSSRb,rNDVI,mND,PSNDa,RARSb和Mcri等也随着温度的升高而降低;Sred随着胁迫加深不断减小,λred向短波方向移动;叶绿素a、叶绿素b和类胡萝卜素含量与光谱反射率及微分光谱,在绿光区和红光区具有显著或极显著的相关关系,且与大部分光谱参数达到极显著相关。说明反射光谱特征及其参数可用来估算叶片色素含量,进而监测毛竹高温受伤害程度。

In order to investigate the relationship between the reflection spectral characteristic and the pigment content of Phyllostachys edulis leaves under the high temperature stress,we measured on the determination to the pigment content and the reflection spectrum of the leaves from the 3-years-old potted seedlings of P.edulis subiected to 25 ℃ （CK）,35 ℃,40 ℃ and 45 ℃ treatments.The relationship between the spectral reflectivity,the first derivative spectrum,and the spectrum parameters were analyzed using the SPSS 13.0 and the OriginPro 8.0 software,respectively.Results showed that：with the increased of temperature,the contents of chlorophyll a,chlorophyll b and carotenoids in the P.edulis leaves decreased,the value of the reflectance spectrum parameters （SR7o5,PSSRa,PSSRb,rNDVI,mND,PSNDa,RARSb and Mcri） also decreased; The red-edge area （Sred） decreased with the enhancement of the temperature stress level,and the position of red-edge inflection （λred） moved to the short-wave direction.There were significant correlations between the content of chlorophyll a,chlorophyll b and carotenoids in the leaves and reflectance spectra characteristics,first derivative of reflectance spectra at the wavelength regions of blue edge and red edge.Moreover,the correlations were extremely significant between pigment contents and almost all spectral characteristic parameters.These reflectance spectrum parameters can be used to evaluate the content of theleaf pigment,and the damage degree of P.edulis by high temperature.