油松成熟胚培养不定芽状态对其生根状况的影响

Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting

油松（Pinus tabulaeformis）是我国特有的针叶树种，木材坚实，富含松脂，耐腐朽，是优良的建筑、电杆、枕木、矿柱等用材。油松是北方的主要造林树种之一，它分布广，适应性强，根系发达，树姿雄伟，枝叶繁茂，有良好的保持水土和美化环境的功能（徐化成，1993）。

The mature embryo of Pinus tabulaeformis was used as explants to induce adventitious buds and root on modified 1/2MS medium. The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces. The rooting rate of adventitious buds was 91.7%. The adventitious bud clusters with diameter larger than 0.9 cm, and the shoots with length longer than 6 mm had higher rooting rate. In the process of shoot elongation and muhiplication, short culture duration could lead to a higher rooting capacity of the adventitious bud cluster, and the 28-days culture period was optimal for rooting, leading to a rooting rate of 73.3%. Adventitious bud clusters with big biomass were easily to root, and the higher the rooting rate the bigger the bud cluster biomass.