急性脑卒中血清神经元特异性烯醇化酶与NO的相关研究

The Interrel Ationresearch Between Neuronspecific Enolase and Nitric Oxide in Serum of Patients with Acute Cerebral stroke

目的 研究神经元特异性烯醇化酶 (NSE)和部分细胞因子在急性脑血管病诊断、病情转归和判断预后的应用价值。方法 应用酶联免疫反应法 (EL ISA)动态测定急性脑血管病病人血清 NSE、NO的水平、变化规律及其相互作用关系 ,利用直线回归分析血清 NSE含量与其他细胞因子的相关性。结果 1脑梗塞和脑出血病人血清 NSE水平显著高于对照组 ,发病后第 1 d最高 ,随着时间的推迟逐渐下降 ,至发病后 2 8d仍明显高于对照组 ,发病后各时间点之间比较无显著性差异。 2脑梗塞和脑出血病人血清NO水平显著降低 ,随着时间的推迟逐渐有升高趋势 ,至发病后 2 8d仍明显低于对照组。结论 NSE、NO可作为急性脑卒中早期诊断、判断病情转归和预后的综合评价指标。

Objective To study the value of the neuron specific enolase and nitric oxide in serum of patients with acute cerebral stroke and the applied of diagnosis,development and outcome in stroke.Methods The level of NSE,NO in serum of patients with acute cerebral stroke were determined by enzyme linked immuno sandwich assy (ΕLISA).The level of NO was determined by colorimetric method.The correlation between the NSE and the NO were analysed by linear corelation regression.Results ①The serum level of NSE of patients with acute cerebral stroke was markly higher than that of controll group,and peaked in 1 d,then decresed for 28 d.②The serum level of NO of patients was lower than that of controll group,and increased eventually for 28d.Conclusion The NSE and NO might serve as indexes of diagnosis,development and outcome in stroke.