脑缺血再灌注胃肠损伤老龄大鼠血浆神经肽的变化及其意义

Changes and significance of neuropeptide in the aged rats with gastrointestinal injury after brain ischemia reperfusion

目的 :从内皮素 (ET)和降钙素基因相关肽 (CGRP)及神经降压素 (NT)的变化方面探讨老龄大鼠脑缺血再灌注胃肠损伤的机制。方法 :青年 (5月龄 )和老龄 (2 0月龄以上 )大鼠均分为模型组和正常对照组 ,观察大鼠全脑缺血 30分钟再灌注 6 0分钟后胃肠组织病理损伤和血浆ET、CGRP、NT含量。结果 :脑缺血再灌注胃肠组织出现明显的病理改变 ,老龄大鼠较青年大鼠严重。青年对照组血浆中CGRP高于青年模型组和老龄对照组。老龄模型组血中ET高于老龄对照组和青年模型组。青年对照组血浆中NT高于老龄对照组和青年模型组。结论 :脑缺血再灌注胃肠损伤与以ET占优势的CGRP与ET的平衡失调和NT含量降低有关 。

Objective: To study the mechanism of gastrointestinal injury after brain ischemia reperfusion in aged rats from the changes of endotheliolysin(ET),calcitonin gene relationed peptide(CGRP),NT. Methods: Young(5 months)and aged (20 months or more)rats were divided into young model group(YMG),young control group(YCG),aged model group(AMG),aged control group(ACG).The following items were measured in rats with 60 minute reperfusion after 30 minute brain ischemia:the level of ET,CGRP,and NT in plasma as well as the morphological changes of gastrointestinal tract. Results: Pathological change of gastrointestinal tract was found distinctly in the YMG and the AMG,this change was seriously in the aged rats than that in the young rats.The plasma CGRP and NT level in the YCG was higher than that in the YMG and the ACG.The ET content was higher in the AMG than that in the ACG and the YMG. Conclusion: The gastrointestinal injury after brain ischemia reperfusion was correlated with the NT decrease and the dysequilibrium between CGRP and ET in which ET was dominant.But because the change of ET,CGRP and NT with aging,these pathological changes had distinctive characteristics in aged rats with brain ischemia compared with that in the young rats.