特发性血小板减少性紫癜患者网织血小板的测定

The measurement of reticulated platelets in patient with idiopathic throbocytopenic purpura

目的 :观察慢性血小板减少性紫癜 (c ITP)患者网织血小板百分率 (RP% )和绝对值水平及其在治疗过程中的变化。方法 :采用噻唑橙染色 ,流式细胞仪检测 6 4例正常人和 47例 c ITP患者的 RP%和 RPs绝对值。结果 :c ITP组 RP%〔(2 9.31± 16 .30 ) %〕明显高于对照组〔(5 .83± 1.81) %〕,RPs绝对值明显低于对照组 ,分别为 (6 .33± 4.5 0 )× 10 9/ L 和 (8.70± 2 .6 0 )× 10 9/ L,分别与对照组比较 ,差异有显著性意义 (P <0 .0 5 ) ;患者在接受治疗后 ,RP%和成熟巨核细胞明显降低 ,产板巨核细胞明显增高 ,分别与治疗前比较 ,差异有显著性意义 (P <0 .0 5 ) ;还观察到 ,在血小板升高前 ,RP%升高达峰值 ,此后随血小板升高 ,RP%逐渐下降。结论 :RP%和 RPs绝对值能反映 c ITP患者骨髓血小板生成活性 ,且

Objective:Reticulated platelets is observed in patient with chronic indiopathic thrombocytopenic purpura (cITP).Method:Using fluorent dye thizole orange (TO),we measured the percent and absolute counts of reticulated platelets (RPs) by flow cytometry in 64 normal and 47 cITP patient. Result:Compared with normal group, the patient with cITP had significant higher RP% and lower absolute count of RPs,P< 0.05 .the RP% of cITP patients significantly decreased after treatment.During the treatment period,we found an increase in the RP% preceding the recovery of platelets count.Conclusion:The percent and absolute count of reticulated platelets can reflex the ability of bone marrow thrombocytopoiesis,and RP% was useful in preceding the recovery of platelets.