VD_3结合蛋白的分离纯化

Separation and Purification of VD_3 Binding Protein

目的 :纯化VD3 结合蛋白 (DBP)。方法 :利用DBP的相对分子质量及电荷性质 ,分别用阳离子交换剂、阴离子交换剂和凝胶过滤等分离目的蛋白 ,SDS PAGE鉴定纯度 ,并进行活性分析。结果 :分离到相对分子质量为 5 80 0 0的蛋白质 ,SDS PAGE呈单一区带。结论 :成功分离纯化出DBP。

Objective To separate and purify VD 3 binding protein(DBP).Methods The protein was purified by chromatographers of SP Sephadex,QAE cellulose,Sepharose 4B,Sephadex G200 according to its characteristics of molecular weight and electric charge.Purity,activity and the concentration of SOP were investigated by SDS PAGE.Result The protein of 58?000 was obtained and was indicated single band by SDS PAGE.Conclusion VD 3 binding protein was separated and purified successfully.