补血Ⅰ号方对化疗药物环磷酰胺致血小板减少动物作用的实验研究

Experimental study of the effects of the Buxue I decoction about the rat thrombocytopenia model caused by cyclophosphamide

目的观察补血Ⅰ号方对化疗药物环磷酰胺致血小板减少小鼠模型的作用。方法用腹腔注射化疗药物环磷酰胺制造小鼠血小板减少模型,随机分为6组(每组10只),分别为补血Ⅰ号方高、中、低剂量(83.4,41.7,20.85g·kg-1)组,对照组(500μg·kg-1注射用重组人白介素-11),模型组和正常组,疗程均为10 d。观察上述各组小鼠血小板计数减少、骨髓巨核细胞计数减少及对脾脏、胸腺、肾上腺脏器系数的影响。结果与模型组相比,高剂量补血Ⅰ号方组可以明显提高小鼠血小板数目(P<0.05);高、中剂量补血Ⅰ号方能明显增加脾和胸腺脏器系数(P<0.05);高、低剂量补血Ⅰ号方可明显提高巨核细胞数目(P<0.05或P<0.01)。结论补血Ⅰ号方能减轻化疗药物环磷酰胺对骨髓巨核细胞的抑制,从而提高血小板数目。

Objective To observe the cyclophosphamide effects of the Buxue Ⅰ decoction on the thrombocytopenia caused by eyclophosphamide （CTX）. Methods Sixty thrombocytopenia models created by intraperitoneal injection of CTX were randomly divided six groups （n = 10 each） ：the Buxue Ⅰ low group （20. 85 g · kg^-1 ） , the Buxue Ⅰ moderate group （41.7 g· kg^-1）, the Buxue Ⅰ high group （83.4 g · kg^-1）, the interleukin- 11 control group, the normal control group and model group. Ten days is a treatment course. The effects about the different quantities on the thrombocytopenia, the megakaryocytes in bone narrow as well as the organ coefficient of spleen, thymus, and adrenal gland were observed. Results Compared with those of the model group, the amount of the platelets were increased in Buxue Ⅰ decoction groups, among which the high group had a significant difference （ P 〈 0. 005 ）. The spleen index and the thymus index both were increased in the Buxue Ⅰ decoction groups, and the thymus index had significant differences between the high and the moderate group. The megakaryocytes were significantly enhanced by the high and low dose of Buxue Ⅰ decoction （P〈0.05, P 〈 0.01 ）. Conclusion The Buxue Ⅰ decoction canmitigate the inhibition of megakaryocytes caused by the CTX and it can contribute to the enhancing immunity.