不同扶正药物及其配伍对SMMC-7721人肝癌细胞的诱导分化作用

EFFECT OF VARIOUS DRUGS AND THEIR COMPAT IBILITY OF STRENGTHENING HEALTHY QI ON INDUCING SMMC-7721 CELL DIFFERENTIAT ION

目的 :探讨不同扶正药物及其配伍对SMMC - 772 1人肝癌细胞的诱导分化作用及其作用机制。方法 :选取临床肝癌治疗常用的益气、温阳、补肾、养阴等扶正治法的代表药物 ,并根据阴阳治则理论将其分别配伍为益气养阴、温阳养阴及补肾 (阳 )养阴等复合方治法 ,分别煎成汤液 ,采取大鼠灌胃给药后分离药物血清的方法制备药物血清 ,作用于体外培养的SMMC - 772 1人肝癌细胞 ,以正常大鼠血清和维甲酸作对照。HE染色观察细胞形态 ;RIA法测甲胎蛋白 (AFP)、环核苷酸 (cAMP) ;ELISA法测白蛋白 (Alb)。结果 :养阴、益气养阴、温阳养阴、补肾养阴组细胞Alb分泌量、cAMP含量增加 ,AFP分泌量降低 ,细胞核浆比例缩小。结论 :养阴药北沙参、麦门冬及其与益气、温阳、补肾配伍后的药物血清具有诱导SMMC - 772 1人肝癌细胞分化的作用 ;提高细胞内cAMP水平 。

Objective:To investigate the effect of various drugs for strengthening healthy Qi and their compatibility on inducing SMMC-7721 hepatocarcinoma cell differ entiation and the mechanism. Methods:some herbs for strengthening healthy Qi,which were often used to tre at live cancer in clinic,such as qi tonics,yang tonics,shen tonics a nd yin tonics were selected and divided into four complex groups accordin g to the Yin-Yang principle,then decocted,administrated the rats via gavage and separared the pharmacal sera for using on SMMC-7721 cells in vitro.Normal se ra of rats and Retinal acid as controls.HE stain method was applied to observe c ell morphosis,AFP and cAMP were measured by RIA,Albumin by ELISA. Results:Albumin secretion and cAMP of the cell increased,AFP secretion decreased in each compatibility group and the ratio of the cell nucleus to plasma decreas ed. Conclusions:The study showed that Yin tonics such as Glehnia Root,Ophio pogon Root and their compatibility Qi,Yang,Shen tonics can remarkably indu ce SMMC-7721 cell differentiation.The mechanism is that the strengthen healthy Qi herbs can increase cAMP,therefore induce hepaotcarcinoma cell differenti ation.