二甲双胍及甘精胰岛素对子宫内膜癌细胞增殖和凋亡的影响

Effect of metformin and glargine on proliferation and apoptosis of human endometrial cancer cell in vitro

目的探讨二甲双胍(Met)、甘精胰岛素(Gla)或二者联合对人子宫内膜癌Ishikawa细胞增殖和凋亡的影响。方法不同浓度Met、Gla或二者联合干预人子宫内膜癌Ishikawa细胞株不同时间。CCK-8检测细胞增殖,流式细胞仪检测细胞凋亡。结果与对照1组比较,Met各浓度均可抑制Ishikawa细胞增殖,小剂量(5mmol/L)可使增殖率下降18.4%。Met可诱导Ishikawa细胞凋亡,高浓度(20mmol/L)晚期凋亡率升高最显著(26.11%)。Gla可促进人子宫内膜癌Ishikawa细胞增殖,细胞凋亡率降低。Gla联合不同浓度Met,与同浓度同时间Gla干预对比,可抑制增殖,增加凋亡率。结论 Met可抑制人子宫内膜癌Ishikawa细胞增殖,促进其凋亡,并可阻断Gla对人子宫内膜癌细胞的促增殖作用。

Objective To investigate the effect of metformin,glargine or drug combination on the proliferation and apoptosis of Ishikawa cell line.Methods Ishikawa cell was cultured in vitro and intervened with metformin,glargine and drug combination for different time.The rate of cell proliferation was tested by CCK-8 assay,and cell apoptosis were detected by flow cytometer.Results Compared with control 1 group,5,10 and 20 mmol/L metformin could inhibit Ishikawa cell line significantly.The rate of proliferation index was reduced 18.4％ by 5 mmol/L metformin intervened.Metformin could induce apoptosis of Ishikawa cell line,and the rate of the most significant late apoptosis increased was 20mmol/L concentration （26.11％）.On the contrary,10,100 and 1000 IU/L glargine could induce the cell proliferation and the rate of cell apoptosis was lower.Compared with the 100 IU/L glargine,which combined with different concentrations metformin had higher cell proliferation and lower apoptosis.Conclusion Metformin can inhibit Ishikawa cell line and induce cell apoptosis in vitro.Metformin can stop the effect of glargine on inducing cell proliferation of Ishikawa cell line.