系统性红斑狼疮患者血清GM-CSF、IL-6和TNF-α的水平测定及临床意义

Levels of GM-CSF, IL-6 and TNF-α in serum of patients with systemic lupus erythematosus

目的检测系统性红斑狼疮(SLE)患者血清中中性粒细胞巨噬细胞集落刺激因子(GM-CSF)、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的水平,探讨其在SLE中的作用。方法选择61例活动性SLE患者,并将其分成轻、中、重三组及抗dsDNA阳性和阴性两类,采用酶联免疫吸附法(ELISA法)测定其血清GM-CSF,IL-6,TNF-α水平,分析其与SLE实验室指标相关性。结果 SLE患者血清GM-CSF,IL-6,TNF-α水平显著高于正常对照组(P<0.05),且和SLE的活动度呈正相关;抗双链DNA抗体阳性患者血清GM-CSF、IFN-α、IL-6水平明显高于阴性者(P<0.05)。SLE患者血清GM-CSF水平与IL-6、TNF-α呈正相关(P<0.05)。结论 SLE患者外周血细胞因子分泌异常可引起免疫系统的紊乱,监测血清细胞因子可有助于了解患者狼疮活动程度,指导临床治疗。

[Objective] To evaluate the serum levels of cytokines（granulocyte-macorphage colony stimulating factor GM-CSF, interleukin6, IL-6, tumor necorsis factor α, TNF-α and explore the pathogenesis of cytokines in theactive systemic lupus erythematosus （SLE）.[Methods] 61 cases with active SLE were graded in three levels （minor,medium, serious） and divided into two groups （anti-dsDNA positive, anti-dsDNA negative）. Serum levels of GM-CSF, IL-6, TNF-α were measured by using ELISA method. [ Results ] The serum levels of GM-CSF, IL-6, TNF-α in active SLE patients were enhanced significantly as compared to the normal control, and the serum levels of cy-tokines had a positive correlation with the active SLE. Furthermore, patients with positive anti-dsDNA antibody hadsignificantly higher serum levels of GM-CSF, IL-6, TNF-α than those with negative anti-dsDNA antibody. Serumlevels of GM-CSF in patients with SLE was positively correlated with IL-6, TNF-α （P 〈0.05）. [ Conclusions ] Theabnormally enhanced cytokines disturb the immunity network and measuring serum cytokines is important in clinictherapy in recognizing the degrees of SLE activity.