不同时期盆栽牡丹叶片RNA的提取研究

Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages

以牡丹品种‘洛阳红’为试材，采用CTAB—LiCl改良法，对小风铃期、大风铃期、圆桃期、平桃期、破绽期、初开期和谢花期的牡丹叶片进行总RNA提取。利用琼脂糖凝胶电泳检测RNA完整性，紫外分光光度计检测RNA纯度。结果表明：7个时期提取的总RNA经电泳均检测到28S、18S和5SrRNA条带，吸光度比值A260／A280均介于1．8～2．0之间，其中大风铃期、圆桃期、平桃期、破绽期和初开期的rRNA条带整齐清晰，并且28SrRNA亮度高于18SrRNA；上述表明CTAB—LiCl改良法适合从牡丹叶片中提取总RNA，且大风铃期、圆桃期、平桃期、破绽期和初开期是最适合提取总RNA的5个时期。

Taking Paeonia suffruticosa Andr. cv. ＇ Luoyanghong ＇ as test material, total RNA were extracted by CTAB- LiCI improved method from tree peony leaves at small bells stage, big bells stage, circular peach stage, flat peach stage, flaw stage,initial bloom stage and fading stage. RNA integrity was detected by agarose gel electrophoresis, RNA purity was detected by ultraviolet spectrophotometer. The results showed that after electrophoresis,the RNA extracted in seven stages all had three bands-28S,18S and 5S rRNA,the ratio of A2G0/A280 ranged between 1.8 and 2. 0. The rRNA bands at big bells stage, circular peach stage, flat peach stage, flaw stage, and initial bloom stage were tidy and clear, and the lightness of 28S rRNA band was higher than 18S rRNA band. The above showed that CTAB-LiCl improved method was suitable for extraction of total RNA from tree peony leaves, and big bells stage, circular peach stage, flat peach stage, flaw stage and initial bloom stage were the optimal five stages for total RNA extraction.