大鼠肝表面移植脾片细胞免疫学研究

The cell immunological study of the transplanted spleen patch on the rats' liver surface

目的 :观察不同时期肝表面移植脾片的细胞免疫学情况以及移植前后脾片量的变化 ,对肝表面脾片移植进行综合评价。方法 :以Wistar大白鼠为研究对象 ,流式细胞仪检测移植脾片的T淋巴细胞总数、活化T细胞数、T辅助细胞数、T抑制细胞数。结果 :8周组CD3+CD8、CD4与对照组比 ,P <0 .0 5 ,4周组与任一组比 ,P <0 .0 1,脾片重量 6～ 8周均明显低于移植前 (P <0 .0 1)。结论 :肝表面移植脾片 6周及 8周各项T淋巴细胞数达到正常脾脏水平 ,脾片重量较移植前有所减轻。脾片成活理想。

Objective:To make a cell immunological study of the patch of the spleen transplanted on the liver surface and the weight of the patch of the spleen in difference period,and evaluate the transplantation of the patch of the spleen on the liver surface.Methods:Wistar rats were used as the investigated object. Flow cytometry was used to examine the transplanted patch of the spleen in terms of the CD3、CD3+CD25、CD4、CD8 and CD4/CD8.Results:Statistical difference was found between 8 week group and control group in CD3+CD25 and CD4(P<0.05) was significantly different. 4 week group and every other group(P<0.01),and the weight of the patch of the spleen in 6～8 week group and control group(P<0.01).Conclusion:All of the T lymphocytes in the patch of the spleen achieve the normal level in 6 to 8 weeks. The weight of the patch of the spleen is lighter than before. The patch of the spleen has desirable survival.