HPLC法测定三子降酶胶囊中五味子醇甲的含量

Determination of Schisandrin in Sanzi Jiangmei Capsules by HPLC

目的建立三子降酶胶囊中五味子醇甲含量的高效液相色谱测定方法。方法采用Agilent Eclipse XDB C18（4．6mm×250mm，5μm）色谱柱，流动相：甲醇-水（63：37），流速：1．0mL·min-1检测波长：250nm，柱温：35℃。结果五味子醇甲在0．10～1．97μg内呈良好的线性关系（r=0．9999），平均回收率为98．4％（n=6），RSD=0．8％。结论本方法简便、准确、重复性好，可用于三子降酶胶囊的质量控制。

Objective To establish a HPLC method for the determination of schisandrin in Sanzi Jiangmei Capsules. Methods Agilent Eclipse XDB C18（4.6mm×250mm,5μm） column was used.The mobile phase was methanol-water （63：37）. The flow rate of 1.0mL·min-1 was adopted. The detection wavelength was 250nm and the column temperature was 35℃. Results The good linear range of schisandrin was 0.10-1.97μg（r=0.9999）.The average recovery of schisandrin（n=6） was 98.4%（RSD=0.8%）. Conclusion This method is simple, accurate and repeatable and can be used for the quality control.