过量表达miR319对番茄果实品质的影响

Effect of Over-Expression miR319 on Fruit Quality of Tomato

[目的]探索过量表达miR319和过量表达LA对番茄果实品质的影响。[方法]以番茄野生型M82、过量表达miR319和过量表达LA番茄为材料,分析过量表达miR319和过量表达LA对番茄果实品质的影响。[结果]过量表达miR319植株的可滴定酸、可溶性糖、VC和番茄红素的含量下降,而过量表达LA植株的可滴定酸、可溶性糖、番茄红素的含量上升,VC含量下降。2个转基因植株可溶性蛋白的含量与M82比较都有所上升。[结论]过量表达miR319和过量表达LA对番茄果实品质的影响是相反的,过量表达LA可以提高番茄果实品质。

[Objective] The present study aimed to explore the effects of over-expression miR319 and over-expression LA on fruit quality of tomato.[Method] Effects of over-expression miR319 and over-expression LA on fruit quality of tomato were analyzed by using tomato wild type M82,over-expression miR319 and over-expression LA as material.[Result] The results showed that：The over-expression jaw-D titratable acid,soluble sugar content VC and Lycopene content were decreased,but over-expression LA,plant titratable,soluble sugar content and Lycopene content were increased,and Vc content was decreased.And two transgenic plants soluble protein content were better than M82.[Conclusion] The effects of over-expression of miR319 and over-expression LA on fruit quality of tomato are opposite and overexpression LA can improve fruit quality.