秦巴山区黑木耳黑色素的提取及抗氧化能力研究

Research of extraction and antioxidant activities of melanin in Auricularia polytricha from Qin-ba mountain area

研究从秦巴山区黑木耳中提取黑色素的最佳工艺条件及其抗氧化能力。采用微波预处理,水浴浸提方法从黑木耳中提取黑色素,在单因素实验的基础上,通过正交实验优化提取工艺。实验结果表明,黑木耳黑色素的最佳提取工艺条件是:NaOH溶液(1.25mol/L)与95%乙醇的体积比为1∶3,水浴温度为60℃,料液比为1∶30(g∶mL),回流时间为70min。抗氧化实验表明:黑木耳中所含黑色素具有较强的抗氧化能力,黑色素浓度达到0.4mg/mL以上时,对过氧化氢清除率达到50%以上;黑色素浓度达到0.18mg/mL以上时对DPPH自由基的清除率超过50%。柠檬酸和酒石酸对抗氧化能力具有一定的协同作用。

The optimum technological condition of extracting melanin from auricularia polytricha and its antioxidantactivities were researched.Melanin was extracted from auricularia polytricha through microwave-pretreatment andwater-bath. The extraction technology was optimized through orthogonal experiment based on single-factorexperiments.The results showed the optimum technological conditions as follows, the volume ratio of 1.25mol/LNaOH-95% ethanol was 1：3, bathing temperature was 60℃, solid-liquid ratio was 1：30,the reflux time was 70min.The experiment of antioxidant showed that the melanin had antioxidant activities, hydrogen peroxide clearance ratewas up to 50% when the melanin＇ s concentration was 0.4mg/mL,and the rate of scavenging free radical DPPHwas up to 50% when the melanin＇ s concentration was 0.18mg/mL, and the citric acid and the tartaric couldenhance the antioxidant activities through the antioxidant experiment.