大越豆芋蛋白ACE抑制肽的分离纯化研究

Study on separation and purification of ACE inhibitory peptides from Apios americana Medikus

本文以大越豆芋为原料,利用酶法制备了大越豆芋低聚肽,研究了其血管紧张素转换酶(angiotensin converting enzyme,ACE)抑制活性。依次通过超滤、大孔吸附树脂脱盐、阴离子交换层析及凝胶色谱法对大越豆芋多肽ACE抑制组分进行了分离纯化。结果表明,酶解液经过超滤处理后,获得ACE抑制率为62.32%的组分IV,该组分通过DA201-C大孔吸附树脂脱盐纯化,得到ACE抑制率为66.65%的豆芋多肽;再采用DEAE-SepHarose Fast Flow阴离子交换树脂分离到组分G3,其IC50值为66.00μg/mL,最后使用SepHadex G-15凝胶分离得到活性较强的组分F2,IC50值可达37.47μg/mL。

In the present paper,Apios americana Medikus root was used as the raw material to produce polypeptideby enzyme hydrolysis method and its ACE inhibitory activity was investigated. To purify the ACE inhibitorycomponent from Apios americana Mediku peptides, the enzyme hydrolysis products were separated in turn byultrafiltration,macroporous resin desalination, anion exchange chromatography and gel chromatography. Resultsshowed that the component IV which had 62.32% ACE inhibitory rate was obtained after the ultrafiltration processof the enzymolysis liquid.After desalinating with DA201-C macroporous resin,a component with a ACE inhibitoryrate of 66.65% was purified from the component IV.The component was further purified with anion exchange resin,DEAE-Sepharose Fast Flow.The component G3 showed highest ACE inhibitory activity with IC 50 value 66.00μg/mL.The component G3 was further separated by using Sephadex G-15 gel chromatography and the component 12was obtained,which showed a high ACE inhibitory activity with a IC50 value of 37.47μg/mL.