响应面法优化可口革囊星虫胶原抗氧化肽的制备研究

Optimization of the preparation of collagen antioxidant peptide of Phascolosoma esculenta by response surface method

本研究对可口革囊星虫抗氧化肽的制备条件进行了优化。通过酶解筛选试验确定最佳用酶为木瓜蛋白酶。在单因素试验基础上确定酶解时间、加酶量和料液比为自变量,以总抗氧化能力(T-AOC)为响应值,通过响应面法分析得出最优条件为酶解时间为4.28h,加酶量为3614U/g,料液比为1∶36,参考实际因素,将提取条件调整为酶解时间4.25h,加酶量3600U/g,料液比1∶36,该条件下实际测得可口革囊星虫胶原抗氧化肽的T-AOC为3.8U/mg。

The preparation conditions of Phascolosoma esculenta antioxidant peptide were optimized.Papain wasoptimum enzyme selected by enzyme filtering experiment.Hydrolysis time ,enzyme amount and ratio of material toliquid were determined by single factor experiment as independent variables, while total antioxidant capacity （T-AOC） resistance as the response value.The optimum solutions for Phascolosoma esculenta antioxidant peptide byresponse surface method were listed as follows：reaction time was 4.28h, and enzyme dosage was 3614U/g, andsolid to liquid ratio was 1：36.Taking operability into account, the extraction conditions were adjusted to： reactiontime was 4.25h, and enzyme dosage was 3600U/g, and liquid ratio was1：36. Under such conditions, thecorresponding T-AOC of the Phascolosoma esculenta collagen peptide was 3.8U/mg.