木薯叶致突变作用研究

Research of mutagenicity of the Cassava leaves

目的:研究木薯叶的致突变作用,为其安全利用提供参考。方法:蚕豆根尖微核试验、小鼠骨髓细胞微核试验及小鼠精子畸形试验。结果:木薯叶浓度为15.80mg/mL以上,蚕豆根尖微核率增加,与阴性对照相比较具有显著性差异(p<0.01),微核指数为1.77;小鼠骨髓微核率增加,与阴性对照相比存在显著性差异(p<0.05,p<0.01);木薯叶浓度为7.90mg/mL以上,对雄性小鼠的精子畸形率增加,与阴性对照比较存在显著性差异(p<0.01)。结论:一定浓度的木薯叶可能对植物细胞、动物体细胞和生殖均存在致突变作用。

Objective：The mutagenic of cassava leaves was researched for its safe to use. Method： The relevanttoxicology tests,including broad bean root tip micronucleus tesVthe micronucleus test of bone marrow cells inmice and sperm abnormality test in mice,were used to study the cassava leaves＇ s poisonous effect.Result： It wasfound that the broad bean root tip micronucleus rate would increase when the concentration of cassava leaves wasabove 15.80mg/mL,the micronucleus index was 1.77,and there was a significant difference between the group ofexperiment and the negative control （ p 〈 0.01 ）. It was discovered that the male and female mice bone marrowmicronucleus rate was increased significantly when the concentration of cassava leaves was above 15.80mg/mL,compared with the negative control （p 〈 0.05, p 〈 0.01 ）. When the concentration of cassava leaves was above7.90mg/mL,it was found that the male mice sperm malformation rate would increase, compared with negativecontrol （ p 〈 0.01 ）.Conclusion ： Deciding from this, a certain concentration of cassava leaves may have some harmful effect on the broad bean root tip cells/somatic cells and reproductive cells in mice.