李子提取物诱导人肝癌HepG2细胞的凋亡及其作用机制

The apoptosis of HepG2 cells induced by plum extract and its mechanism

探讨李子提取物对人肝癌细胞HepG2增殖和凋亡的影响及其作用机制。采用Alamar Blue法检测李子提取物对HepG2细胞增殖的抑制作用;倒置显微镜、Hoechst 33258荧光染色、流式细胞术检测李子提取物对HepG2细胞凋亡的影响;Western blot分析李子提取物诱导HepG2细胞凋亡的作用机制。结果表明,5%和10%的李子提取物作用HepG2细胞48h后,明显抑制HepG2细胞的增殖;细胞出现明显的凋亡特征,流式细胞术进一步证实李子提取物能够诱导HepG2细胞的凋亡;处理组HepG2细胞中Bcl-2和Survivin表达量降低,p53,Bax,Bid,Caspase-9和Caspase-3表达量增加并且Caspase-3酶活性显著提高。李子提取物通过内源性凋亡信号通路诱导HepG2细胞的凋亡发挥其抗肿瘤活性。

The objective of this study was to evaluate the effect of plum extract on the proliferation and apoptosis ofHepG2 cells and its mechanism. The proliferation of HepG2 cells was examined by Alamar Blue assay. Theapoptosis of HepG2 cells was determined by inverted fluorescence microscope, Hoechst 33258 and flow cytometryanalysis.Western blot analysis was used to detect the expression of the apoptosis-related factors in HepG2 cells.Results indicated that the cell proliferation was inhibited by plum extract in a dose dependent manner andmorphologic alterations of cells were observed after treated with plum extract for 48h.The results of flow cytometryanalysis showed that the apoptotic ratio of HepG2 cells treated with plum extract was significantly increasedcompared with control.Plum extract down-regulated the expression of Survivin,Bcl-2 and up-regulated the p53,Bax,Bid,Caspase-9 and Caspase-3 in HepG2 cells. Besides, the Caspase-3 activity were also increased intreated group. It provided evidence that plum extract induced the apoptosis of HepG2 through endogenousapoptosis signaling pathway.