石榴皮醇提物的急性肝毒性研究

Hepatotoxicity of Pomegranate Peel Ethanol Extract in Vivo

目的评价石榴皮醇提物对小鼠肝脏的影响。方法将40只小鼠随机分成阴性对照组、石榴皮醇提物低、中、高剂量组(30,45,60 mg·kg-1)。所有小鼠在禁食12 h后,各剂量组给予相应剂量的石榴皮醇提物,阴性对照组给予等体积的蒸馏水。给药24 h后,所有小鼠麻醉后眼眶取血,麻醉脱臼处死。测定ALT,AST,LDH血清酶指标。留取肝组织测定MDA、GSH、GSSG评价氧化损伤的指标。留取1/2肝大叶经福尔马林溶液固定,HE染色观察小鼠肝脏病理改变。结果与阴性对照组比较,石榴皮醇提物中、高剂量组体质量、肝重、肝体比明显降低(P<0.05或P<0.01),ALT、LDH、MDA明显升高(P<0.05),GSH/GSSG明显降低(P<0.05),石榴皮醇提物高剂量组AST明显升高(P<0.05)。结论石榴皮醇提物能够引起小鼠肝脏的急性损伤,并且剂量越大,毒性越大。

OBJECTIVE To evaluate the hepatotoxicity of pomegranate fruit peel ethanol extract in mice. METHODS An experimental study was carried out in mice. After random allocation of the intervention, forty mice were received pomegranate fruit peel ethanol extract（30, 45, 60 mg·kg^-1） or distilled water while in a fasted state for 12 h. After 24 h, the level of serum enzyme was measured with automatic biochemistry analyzer, which included ALT, AST, and LDH. MDA, GSH and GSSG were measured in liver homogenates to assess the oxidative damage. Histological changes in liver were observed by HE staining. RESULTS Compared with the control group, there were significant decreases of body weight, liver weight, liver weight and body weight ratio, significant increases of ALT, LDH, MDA, GSH/GSSG in both the middle and high dose of pomegranate fruit peel ethanol extract groups（P〈0.05）, and significant increase of AST in the high dose group. CONCLUSION These results indicates that oral administration of pomegranate fruit peel ethanol extract afford statistically significant toxicity to mice liver, and thus, the higher the dose, the more serious liver damage.